Molecular mechanism for the inhibition of photosynthesis by UV and excess photon energy and its proteshon

紫外线和过剩光子能量抑制光合作用的分子机制及其蛋白质

• 批准号: 12440230
• 负责人: ASADA Kozi
• 金额: $ 2.05万
• 依托单位: Fukuyama University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-12440230/
• 关键词: ultra-violet light; ercess photon energg; singlet oxggen; watev-water cycle; 3eta-crystallin; photoinhibition; blue-grean flworescauce; ascorbate peroxidase; ゼータクリスタリン; 青緑蛍光; アスコルビン酸ペルオキシダーゼ; 紫外光エネルギー; 過剩光エネルギー; Water-Waterサイクル; ゼーター・クリスタリン様

The sunlight is essential for plant growth, but its UV components inhibit photosynthesis. Even the photosynthetically-essential visible components also produces reactive species of oxygen (ROS) and causes the photoinhibition, when leaves are exposed to sunlight intensities in excess of that required for CO_2-fixation. In the present research, the molecular mechanisms of photoinhibition and its protection have been studied on the following topics. 1) Ascorbate peroxidase is the primary target molecules of ROS to induce photoinhibition, 2) Enzymatic properties and physiological functions of zeta-crystallin protein of plants, 3) Monodehydroascorbate reductase is able to catalyze also the reduction of phenoxyl radicals, 4) Induced biosynthesis of blue-green fluorescence components by UV irradiation and its cellular localization, 5) Characteristic properties of the cyclic electron transport around PSI, as revealed by redox change of P700, 6) Detection of singlet oxygen photoproduced in leaf cells, 7) Physiological functions of the water-water cycle in chloroplasts and Zooxanthelle.

阳光对植物生长至关重要，但其紫外线成分抑制光合作用。当光照强度超过CO_2固定所需强度时，即使是光合作用所必需的可见光组分也会产生活性氧（ROS），引起光抑制。本研究围绕以下主题研究了光抑制及其保护的分子机制。1)抗坏血酸过氧化物酶是ROS诱导光抑制的主要靶分子，2）植物zeta-晶体蛋白的酶学性质和生理功能，3）单脱氢抗坏血酸还原酶也能够催化苯氧基自由基的还原，4）通过UV照射诱导蓝绿色荧光组分的生物合成及其细胞定位，5）PSI周围的循环电子传递的特征，如通过P700的氧化还原变化所揭示的，6）叶细胞中光产生的单线态氧的检测，7）叶绿体和虫黄质中水-水循环的生理功能。

项目成果

浅田浩二: "植物の葉はどうして太陽光の下で緑を保っているのか?"マテリアル学会誌. 13. 114-122 (2001)

Koji Asada：“为什么植物叶子在阳光下保持绿色？”材料科学学会杂志 13. 114-122 (2001)

Tomonori Kawano: "Phenyethylamine-induced generation of reactive oxygen spectes and ascoroate free radicals in Tobacco suspension culture"Plant Cell Physiol.. 41. 1259-1266 (2000)

Tomonori Kawano：“烟草悬浮培养物中苯乙胺诱导的活性氧和抗坏血酸自由基的产生”植物细胞生理学.. 41. 1259-1266 (2000)

Mano, J., Ohno, C.et al.: "Choloroplastic ascorhate peroxides is the primary target of methylviologen-lnduced photoox dative stress"Biochim.Biophys.Acta. 1504. 275-287 (2001)

Mano, J., Ohno, C.等人：“氯代抗坏血酸过氧化物是甲基紫精诱导的光氧应激的主要目标”Biochim.Biophys.Acta。

Mano, J. Belles-Boix, E. Babiychuk, E. Asada, K. Van Montagu, M. Inze, D. Kushnir, S. and Slooten, L: "Overexpressed P1-crystallin, a putative antioxidant protein, constitutes an oxygen-dependent alternative electron sink in leaves"Plant physiol. (submitt

Mano, J. Belles-Boix, E. Babiychuk, E. Asada, K. Van Montagu, M. Inze, D. Kushnir, S. 和 Slooten, L：“过度表达的 P1-晶状体蛋白（一种推定的抗氧化蛋白）构成了氧

Asada, K.: "The water-water cycle as altemative photou and election sinks"Phil. Trans. Roy. Soc. London. B. 355. 1419-1431 (2000)

Asada, K.：“水-水循环作为替代光和选举汇”Phil。