Rapid and sensitive measurement of dissolved gases by permeable membrane mass-spectrometry.
通过渗透膜质谱法快速、灵敏地测量溶解气体。
基本信息
- 批准号:63860013
- 负责人:
- 金额:$ 5.38万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Developmental Scientific Research
- 财政年份:1988
- 资助国家:日本
- 起止时间:1988 至 1989
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Flash induced oxygen evolution was measured using a permeable membrane-mass spectrometer. Evolved gases were introduced through a gas-permeable membrane (0.024 mm thick, silicone rubber; General Electric, USA) to a quadrupole mass analyzer (MSQ 15OA; ULVAC, Japan). The paper disk of the lyophillized PS II membranes was gently immersed in 0.3-1.5 ml of 50 mM Hepes/NaOH (pH 6.8) which had been flushed with Ar gas. The reaction chamber (1 cm in diameter) was closed with a gas-tight, acrylic piston lid. After incubation in the dark for 5 to 10 min at 25゚C, the oxygen evolution was measured at the ion peak of 32 m/e under a Xe flash (30-50 J, t_<1/2> = 0.03 ms; Sugawara Kenkyusho, Japan) at dark intervals of 20-30 s. The half-decay time of the signal of the flash induced oxygen evolution was about 2 s when the thickness of the sample solution was 0.1 mm. The oxygen-evolving activity of the resuspended lyophillized PS II membranes was 0.27 mmol of oxygen (mg Chl)^<-1>h^<-1> under continuous … More light which was 70% that of the control PS II membranes. We examined the effect of lyophillization on the oscillation pattern of oxygen evolution by flash illuminations. The oxygen evolution of the control PS II membranes was maximal at the third flash and oscillated with a period of 4 flashes. The lyophillized PS II membranes which were immersed in Hepes buffer, however, showed the maximal evolution of oxygen at the sixth flash, indicating the water-oxidizing enzyme was apparently reduced to "S_<-2> state" by lyophillization. After preillumination the oxygen evolution of the lyophillized membranes oscillated as the control membranes did. Moreover, the miss-hit and double-hit probabilities and S_1/(S_0+S_1) for the oxygen evolution pattern of the lyophillized and preilluminated PS II membranes were similar to those of the control membranes. The lyophillization reduces the water-oxidizing enzyme, and the two photons return the enzyme to the S_0 state only in the presence of the water bound the the high-affinity sites in the enzyme. Less
闪光引起的析氧使用渗透膜质谱仪测量。通过透气膜(0.024 mm厚,硅橡胶;通用电气,美国)将放出的气体引入四极质量分析仪(MSQ 15 OA; ULVAC,日本)。将冻干的PS II膜的纸盘轻轻浸入0.3- 1.5ml的50 mM Hepes/NaOH(pH 6.8)中,其已经用Ar气冲洗。反应室(直径1cm)用气密的丙烯酸活塞盖封闭。在25 ℃下于黑暗中孵育5 - 10 min后,在20-30 s的黑暗间隔下,在30-50 J,t_<1/2> = 0.03 ms; Sugawara Kenkyusho,Japan的闪光灯下,在32 m/e的离子峰处测量氧释放。当样品溶液的厚度为0.1 mm时,闪光诱导的氧释放信号的半衰减时间约为2 s。在连续条件下,再悬浮的冻干PS II膜的氧释放活性为0.27 mmol氧(mg Chl)·<-1>h·<-1>。 ...更多信息 光,这是70%的控制PS II膜。我们研究了冷冻干燥对氧气释放振荡模式的影响。对照PS II膜的氧释放在第三次闪光时最大,并且以4次闪光的周期振荡。而在Hepes缓冲液中浸泡的PS Ⅱ膜在第6次闪蒸时放出的氧最多,表明水氧化酶在冻干过程中明显还原到S<-2>态。预照射后,冻干膜的析氧量与对照膜一样振荡。此外,预光照和冻干PS II膜的失命中概率和双命中概率以及析氧模式的S_1/(S_0+S_1)与对照膜相似。冷冻干燥降低了水氧化酶,并且只有在酶中的高亲和力位点存在结合水的情况下,两个光子才使酶返回到S_0状态。少
项目成果
期刊论文数量(30)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Takahashi, M., Asada, K.: "Molecular structure of a high-potential form of thylakoid cytochrome b-559 as determined by radiation inactivation." Plant and Cell Physiology 30, 915-921, 1989.
Takahashi, M., Asada, K.:“通过辐射灭活测定的类囊体细胞色素 b-559 高潜力形式的分子结构。”
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Takahashi,M: "Molecular structure of a high-potential from of thylakoid cytochrome b-559 as determined by radiation inactivation" Plant and Cell Physiology. 30. 915-921 (1989)
Takahashi,M:“通过辐射灭活测定的类囊体细胞色素 b-559 的高潜力分子结构”植物和细胞生理学。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Ikeda,T.: "Electrocatalytic Photolysis of water at photosystem II modified carbon paste electrode containing dimetylbenzoquinone." Chemistry Letters. 1989. 913-916 (1989)
Ikeda,T.:“在含有二甲基苯醌的光系统 II 改性碳糊电极上对水进行电催化光解。”
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
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ASADA Kozi其他文献
ASADA Kozi的其他文献
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{{ truncateString('ASADA Kozi', 18)}}的其他基金
Development of Assay Method of Singlet Excited Oxygen in Chloroplasts and Molecular Mechanisms of Its Generation, Scavenging and Action
叶绿体中单线态激发氧测定方法及其产生、清除和作用分子机制的建立
- 批准号:
15370026 - 财政年份:2003
- 资助金额:
$ 5.38万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Molecular mechanism for the inhibition of photosynthesis by UV and excess photon energy and its proteshon
紫外线和过剩光子能量抑制光合作用的分子机制及其蛋白质
- 批准号:
12440230 - 财政年份:2000
- 资助金额:
$ 5.38万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Molecular mechanism for the dissipation of excess photon energy in chloroplasts
叶绿体中多余光子能量消散的分子机制
- 批准号:
10440243 - 财政年份:1998
- 资助金额:
$ 5.38万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Isolation and Analysis of Physiological roles of thylakoid-bound NAD (P) H dehydrogenase
类囊体结合 NAD (P) H 脱氢酶的分离和生理作用分析
- 批准号:
06454014 - 财政年份:1994
- 资助金额:
$ 5.38万 - 项目类别:
Grant-in-Aid for General Scientific Research (B)
Mechanism for Relaxation of Photooxidative Stress in Plants.
植物光氧化应激的缓解机制。
- 批准号:
05044130 - 财政年份:1993
- 资助金额:
$ 5.38万 - 项目类别:
Grant-in-Aid for international Scientific Research
Molecular mechanism of NAD(P)H -cyclic electron flow
NAD(P)H-循环电子流的分子机制
- 批准号:
04454014 - 财政年份:1992
- 资助金额:
$ 5.38万 - 项目类别:
Grant-in-Aid for General Scientific Research (B)
Molecular Mechanism of Adaptation to photo-Oxidative Stresses in Cyanobacteria.
蓝藻适应光氧化应激的分子机制。
- 批准号:
02454011 - 财政年份:1990
- 资助金额:
$ 5.38万 - 项目类别:
Grant-in-Aid for General Scientific Research (B)
Adaptation mechanism of plant leaves to photooxidative stresses
植物叶片对光氧化胁迫的适应机制
- 批准号:
02044084 - 财政年份:1990
- 资助金额:
$ 5.38万 - 项目类别:
Grant-in-Aid for international Scientific Research
Action Mechanism of Suicide Inhibitors of Ascorbate Peroxidase
抗坏血酸过氧化物酶自杀抑制剂的作用机制
- 批准号:
02556012 - 财政年份:1990
- 资助金额:
$ 5.38万 - 项目类别:
Grant-in-Aid for Developmental Scientific Research (B)
Molecular adaptation to photo-oxidative stresses in plants.
植物对光氧化胁迫的分子适应。
- 批准号:
63480014 - 财政年份:1988
- 资助金额:
$ 5.38万 - 项目类别:
Grant-in-Aid for General Scientific Research (B)
相似海外基金
X-ray Crystallographic Studies on Mechanism of Photosystem II Membrane Protein Complex
光系统II膜蛋白复合物机理的X射线晶体学研究
- 批准号:
16087102 - 财政年份:2004
- 资助金额:
$ 5.38万 - 项目类别:
Grant-in-Aid for Scientific Research on Priority Areas