Development of Novel Separation Media Using Photo-functioning Host Compounds

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In inventions of new functional chemical substance, elucidation of the biochemical or biological mechanisms or search for the environmental pollutants, etc., it is a column, thin layer or liquid chromatography, which are indispensable separation methods.In these separation methods, organic solvent is often used as extractant, or mobile phase additives in order to adjust the separation characteristics. There are many solvents of being noxious for the ecosystem. In this study, separation technique which could changes the characteristics of the stationary phases instead of the characteristics of mobile phase was demonstrated. It is safe because changing the property of stationary phase itself by external field (light), and that it is also gentle for the environment.In our typical case, γ- cyclodextrin (CD) was made to be the photoresponsivity molecular recognition position, and the packings (the AzCD silica ) which was bonded in the silica gel was prepared. The separation characteristics and the responsibility for ultraviolet and visible light were examined using reversed-phase micro liquid chromatography. Seven kinds of polycyclic aromatic hydrocarbons were used as model samples. When this packing was irradiated with the ultraviolet light, the retention times of the sample molecules eluting earlier, such as the pentacene, showed comparatively small decrease of retention, and the retention time of the molecule with wide peak width, such as perylene, etc. showed reversely increased retention.