GC-atmospheric pressure laser ionisation-HR-time-of-flight mass spectrometer with FF-TG-GC option

通过现有的气相色谱-大气压激光电离-高分辨率飞行时间质谱仪（GC-APLI-TOFMS）和高效液相色谱（LC）选项（2010年资助），开发了分析多环芳烃（PAH，环境污染物）的方法，与普通GC-MS PAH分析相比，其灵敏度约为1，000倍。据我们所知，PAH的检测下限尚未公布。由于成功的方法开发以及LC的额外替代用途，ANOM-MS系统现在可用于不同应用的全部容量。通过GC或LC-APLI-TOFMS分析大气颗粒物（PM）、烟尘、土壤、沉积物、技术基质、室内空气、地表水、海水、地下水等中的PAH，并测量尿液中的PAH代谢物。在合作中，还研究了选定的PM和沉积物的毒性效应。此外，在最古老的前寒武纪岩石中首次检测到芳香族类固醇，这在以前是不可能的。其他应用领域包括地表水和河岸过滤中的农药和药物（目标、非目标和协同效应导向分析），以及用于测定黑碳状结构的苯多羧酸分析。特别是，GC-APLI-TOFMS方法由于其选择性而代表了PAH分析的里程碑，并可能在未来通过开发具有成本效益的紧凑系统来取代常见的PAH分析。在这里，作为第一步，将研究通过流场-温度梯度-GC（FF-TG-GC）的加速色谱法的适用性以及所使用的KrF准分子激光器与DPSS激光器的可比性。作为第二种方法的发展与全面的工作的硬件的仪器，一个效果导向的GC-APLI-飞行时间质谱与回收LC和微型EROD测定计划。通过这种方式，选择性地获得有关PAH和毒性作用的平行信息。使用现有的APLI-MS系统，无法再进行新方法的开发和硬件方面的全面工作，因此，在此应用了第二个相同的GC-APLI-TOFMS系统，并提供额外的FF-TG-GC选项。

By the available gas chromatography-atmospheric pressure laser ionization – high resolution time-of-flight mass spectrometer (GC-APLI-TOFMS) with high performance liquid chromatography (LC) option (funded in 2010), methods for the analysis of polycyclic aromatic hydrocarbons (PAH, environmental pollutants) were developed which are more sensitive by a factor of about 1,000 compared to common GC-MS PAH analysis. To our knowledge, lower limits of detection for PAH have not yet been published. As a result of the successful method developments and due to the additional alternative use with LC, the APLI-MS system is now used to full capacity for the different applications. PAH are analyzed by GC- or LC-APLI-TOFMS in atmospheric particulate matter (PM), soots, soils, sediments, technogenic substrates, indoor air, surface water, sea water, ground water, etc. and PAH metabolites are measured in urine. In cooperation, toxic effects were additionally studied in selected PM and sediments. Furthermore, aromatic steroids were detected for the first time in oldest precambrian rocks, where this had not been possible before. Other application fields are pesticides and pharmaceuticals (target, non-target and in cooperation effect-directed analysis) in surface water and river bank filtration as well as benzenepolycarboxylic acid analysis for the determination of black carbon-like structures. Particularly, the GC-APLI-TOFMS method represents a milestone in PAH analysis due to its selectivity and may replace common PAH analysis by development of a cost-efficient compact system in the future. Here, as a first step, the suitability of an accelerated chromatography by flowfield-temperature gradient-GC (FF-TG-GC) and the comparability of the used KrF eximer laser with a DPSS laser will be studied. As a second method development with comprehensive work on the hardware of the instrument, an effect-directed GC-APLI-TOFMS with recycling-LC and mini EROD-assay is planned. By this, parallel information selectively about PAH and toxic effects are gained. With the available APLI-MS system, new method developments with comprehensive work on hardware is not possible anymore, therefore, a second identical GC-APLI-TOFMS system with an additional FF-TG-GC option is applied for here.