Rice proteome analysis as a post-genome project research

为了有效地分析水稻蛋白质组，本研究发展了多种分析技术。这些技术包括蛋白质凝胶内消化，肽图谱，质谱鉴定的蛋白质分离的二维电泳，鸟枪分析的蛋白质分离的SDS凝胶电泳，检测磷酸化蛋白质，电泳的高分子量蛋白质，蛋白质-蛋白质相互作用分析与一种新的蛋白质芯片。利用该技术对水稻种胚蛋白质组进行了全面的分析。在这项分析中，我们鉴定了许多功能未知的蛋白质。为了获得这些蛋白质的功能信息，我们对它们进行了表达谱分析，并鉴定了几种与发芽相关的蛋白质。另一方面，我们分析了大型蛋白质复合物26 S蛋白酶体，以确定蛋白质翻译后修饰的作用，以获得蛋白质组分析的基础信息。将双向电泳图谱和单个胚胎蛋白质的特征编入我们的蛋白质组数据库。

To analyze efficiently the rice proteome, we developed various analytical techniques in the present study. These include techniques for protein in-gel digestion, peptide mapping, mass spectrometry for identification of proteins separated by two-dimensional electrophoresis, shot-gun analysis of proteins separated by SDS gel electrophoresis, detection for phosphorylated proteins, electrophoresis for high molecular weight proteins, and protein-protein interaction analysis with a novel protein chip. Using the developed techniques, we analyzed comprehensively the rice seed embryo proteome. In this analysis, we identified many proteins without known functions. To obtain the information of functions of these proteins, we performed expression profiling of them, and we identified several proteins, which expressed in relation to germination. On the other hand, we analyzed a large protein complex, 26S proteasome to determine the roles of protein post-translational modifications to obtain fundamental information for proteome analysis. The two-dimensional electrophoresis pattern and the characteristics of individual embryo protein were compiled in our proteome database.