Network for the localization of membrane and secretory proteins in Bacillus srubtilis.

用于定位芽孢杆菌中膜和分泌蛋白的网络。

• 批准号: 12460037
• 负责人: YAMANE Kunio
• 金额: $ 6.85万
• 依托单位: University of Tsukuba
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-12460037/
• 关键词: Bacillus subtilis; Protein secretion machinery; SRP; SecA; Membrane proteins; Proteome; タンパク質分泌機構; on membrane digestion法; PVDFメンフレン; MARDI-TOF MS; 分泌タンパク質; タンパク質の局在化; ネットワーク; シグナルペプチド

Bacillus subtilis and related bacteria has the ability to produce high levels of extracellular enzymes and they have been used as producers of the enzymes in industries. To analyze the molecular mechanism for the protein secretion machinery to produce the enzymes and a possible network between the protein secretion and the localization of proteins in the cell membrane, we have studied as follows1)Protein composition and function of each component of signal recognition particle (SRP) that targets the presecretory proteins to the cell membrane2)Interaction between FtsY, a receptor of SRP, and Ffh, a component of SRP3)Comprehensive analysis of membrane proteins and their relation to the protein secretory machinery to localize the proteins into the cell membrane

枯草芽孢杆菌和相关细菌能够产生高水平的胞外酶，它们已经被用作工业中的酶的生产者。为了分析蛋白质分泌机制产生酶的分子机制，以及蛋白质分泌与细胞膜蛋白质定位之间可能的网络，我们研究了以下内容：1)信号识别颗粒(SRP)每个组成部分的蛋白质组成和功能；2)SRP受体FtsY和SRP成分FFH之间的相互作用；3)膜蛋白的综合分析及其与蛋白质分泌机制的关系，以便将蛋白质定位到细胞膜