权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Molecular cloning and analysis of requlatory-strucutral genes for arginine biosynthtic pathway in Bacillus amyloliquefaciens

解淀粉芽孢杆菌精氨酸生物合成途径调节结构基因的分子克隆与分析

基本信息

批准号：
06044248
负责人：
YAMANE Kunio
金额：
--
依托单位：
University of Tsukuba
依托单位国家：
日本
项目类别：
Grant-in-Aid for Overseas Scientific Survey.
财政年份：
1994
资助国家：
日本
起止时间：
1994 至无数据
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-06044248/
关键词：
Arg Genes Arginine Biosynthetic Pathway Bacillus Amyloliquefaciens

项目摘要

As a model system to compare with those of Escherichia coli, the structure of the arg genes and their molecular genetic property in Bacillus amyloliquefaciens will be investigated. In the case of E.coli, other genes except for the argECBH cluster for the biosynthesis of arginine from glutamate are not tightly linked on the chromosome. Their expressions are also requlated by the product of unlinked gene, argR.The structure of the arg genes and the operon in E.coli has been studied by many researchers. Although the study on the arg auxotroph and the biosynthesis of arginine have been done a little in other microorganisms, molecular genetics of arg genes in Bacillus sp.remains to be elucidated. In professor Min's laboratory the argEC genes and the arg operon in Corynebacterium glutamicum has been cloned and studied. Therefore, the arg genes in Bacillus amyloliquefaciens will be cloned by a complementation analysis of E.coli arg auxotropic mutant. After the nucleotide sequence analysis, the gene organization and requlation of the arg operon in the B.amyloliquefaciens chromosome will be compared with those of E.coli and Corynebacterium system.

作为与大肠杆菌相比较的模型系统，我们将对解淀粉芽孢杆菌中arg基因的结构及其分子遗传特性进行研究。以大肠杆菌为例，除了用于从谷氨酸生物合成精氨酸的argECBH基因簇外，其他基因在染色体上的连接并不紧密。它们的表达也受非连锁基因argR的产物调控。大肠杆菌中arg基因和操纵子的结构已被许多研究者研究过。尽管在其他微生物中对精氨酸的营养不良和精氨酸的生物合成的研究较少，但芽孢杆菌中精氨酸基因的分子遗传学仍有待阐明。闵教授实验室克隆并研究了谷氨酸棒状杆菌的argEC基因和arg操纵子。因此，解淀粉芽孢杆菌中的arg基因将通过大肠杆菌arg突变体的互补分析进行克隆。通过核苷酸序列分析，将解淀粉芽孢杆菌染色体上实面操纵子的基因组织和调控与大肠杆菌和棒状杆菌系统进行比较。