Controlling plasma leptin level improves quality and productivity of beef

控制血浆瘦素水平可提高牛肉的质量和生产率

• 批准号: 12460124
• 负责人: YANO Hideo
• 金额: $ 7.68万
• 依托单位: KYOTO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-12460124/
• 关键词: bovine; leptin; adipocyte; beef marbling; estrogen; genistein; vanadium; leptin secretion and mRNA expression; 脂肪組織; 脂肪細胞径; 硫酸バナジル; リアルタイムPCR; インスリン; PI3-キナーゼ

(1)We studied the changes of plasma leptin concentration during fattening in Japanese Black steers and heifers. We also examined the relationship between plasma leptin level and carcass traits. Plasma leptin concentration was increased during fattening period in the steers and heifers. Although there is no great difference between heifers and steers in the thickness of subcutaneous fat or beef marbling, plasma leptin concentration was higher in the heifers than in the steers. The steers showed the significant correlation between plasma leptin level just before slaughter and the thickness of subcutaneous fat or beef marbling that probably reflected body fat content. These results suggested that plasma leptin level possibly used as the index for the degree of fattening in the steers. On the other hand, plasma leptin level did not related to these carcass traits in the heifers.(2)We investigated leptin mRNA expression, adipocyte size, and their relationship in several adipose tissues of f … More attening steers. The intramuscular adipose tissues had a lower leptin mRNA level than the intermuscular and perirenal adipose tissues. The leptin mRNA level was lower in the subcutaneous depot than in the intermuscular depot. The adipocyte diameter was larger in the intermuscular adipose tissue than in the subcutaneous and the intramuscular adipose tissues. Leptin mRNA level was positively correlated to adipocyte diameter.(3)We investigated the influence of estrogen, sex steroid hormone, and genistein, soybean isoflavone, on leptin secretion and mRNA expression in bovine adipocytes. We also examined the effect of vanadium on leptin mRNA expression. Estrogen and genistein stimulated in leptin secretion from bovine adipocytes after 2 and 8 h of treatment. On the other hand, mRNA levels for leptin were increased by estrogen and genistein after 6 and 24 h of treatment, though 2 h of treatment did not significantly affect leptin mRNA synthesis. Low concentration of vanadium increased leptin gene synthesis, while high concentration decreased leptin expression prominently. Less

(1)研究了日本黑牛和小母牛育肥过程中血浆瘦素浓度的变化。我们还研究了血浆瘦素水平与胴体性状之间的关系。阉牛和小母牛的血浆瘦素浓度在育肥期间增加。尽管小母牛和小公牛的皮下脂肪厚度或牛肉大理石花纹没有太大差异，但小母牛的血浆瘦素浓度高于小母牛。阉牛显示出屠宰前血浆瘦素水平与皮下脂肪或牛肉大理石花纹的厚度之间存在显着相关性，这可能反映了身体脂肪含量。这些结果表明，血浆瘦素水平可作为肉牛育肥程度的指标。另一方面，血浆瘦素水平与小母牛的这些胴体性状无关。(2)我们研究了瘦素 mRNA 表达、脂肪细胞大小及其在几种精育公牛脂肪组织中的关系。肌内脂肪组织的瘦素 mRNA 水平低于肌间和肾周脂肪组织。皮下贮库中的瘦素 mRNA 水平低于肌间贮库中的瘦素 mRNA 水平。肌间脂肪组织中的脂肪细胞直径大于皮下和肌内脂肪组织中的脂肪细胞直径。瘦素mRNA水平与脂肪细胞直径呈正相关。(3)研究了雌激素、性类固醇激素、染料木黄酮、大豆异黄酮对牛脂肪细胞瘦素分泌和mRNA表达的影响。我们还研究了钒对瘦素 mRNA 表达的影响。治疗2小时和8小时后，雌激素和金雀异黄素刺激牛脂肪细胞分泌瘦素。另一方面，雌激素和金雀异黄素治疗 6 小时和 24 小时后，瘦素 mRNA 水平增加，但治疗 2 小时并没有显着影响瘦素 mRNA 合成。低浓度的钒增加了瘦素基因的合成，而高浓度的钒则显着降低了瘦素的表达。较少的

项目成果

Hiroyuki Kawachi: "Vanadium Stimulates leptin secretion from rat adipocytes"Japan Trace Nutrients Research Society. 18. 41-45 (2001)

Hiroyuki Kawachi：“钒刺激大鼠脂肪细胞分泌瘦素”日本微量营养素研究会。

Seung Hak Yang: "Fat depot-specific differences in leptin mRNA expression and its relation to adipocyte size in steers"Animal Science Journal. 74・1. 17-21 (2003)

Seung Hak Yang：“瘦素mRNA表达的脂肪库特异性差异及其与脂肪细胞大小的关系”动物科学杂志74・1（2003）。

Tomomi Tokuda: "Blood leptin concentration in Japanese Black cattle"Animal Science. 72・2. 309-313 (2001)

德田朋美：“日本黑牛的血液瘦素浓度”动物科学72・2。

Tomomi Tokuda: "Blood leptin concentrations in Japanese Black cattle"Animal Science. 72-2. 309-313 (2001)

Tomomi Tokuda：“日本黑牛的血液瘦素浓度”动物科学。

河内 浩行: "バナジウムはラット脂肪細胞からのレプチン分泌を促進する"微量栄養素研究. 18. 41-45 (2001)

Hiroyuki Kawachi：“钒促进大鼠脂肪细胞分泌瘦素”微量营养素研究 18. 41-45 (2001)。