课题基金 / 基金详情

Physiological significance of metallothionein on environmental carcinogenesis

Physiological significance of metallothionein on environmental carcinogenesis
金属硫蛋白对环境致癌的生理意义
批准号:
12470090
负责人:
TOHYAMA Chiharu
金额:
$7.94万
依托单位国家:
日本
项目类别:
Grant-in-Aid for Scientific Research (B)
财政年份:
2000
资助国家:
日本
项目状态:
已结题
起止时间:
2000 至 2002

项目摘要

项目成果

TOHYAMA Chiharu的其他基金

相似基金

相关文献

中文摘要
翻译
为探讨金属硫蛋白(MT)在环境致癌中的作用,本研究观察了MT对7,12-二甲基苯并[a]蒽(DMBA)/12-O十四酰佛波醇-13-乙酸酯(TPA)诱发的两阶段皮肤癌的影响,采用MT基因敲除小鼠,观察DMBA诱导的多器官癌变和N-乙基-N-亚硝基脲(ENU)诱导的肺癌的发生,II,MT的主要亚型,被抑制。此外,我们还检测了MT基因敲除小鼠对DMBA/TPA、苯并[a]芘(B[a]P)或X射线引起的遗传毒性的敏感性,发现MT基因敲除小鼠对DMBA/TPA组合引起的皮肤肿瘤的发生率、DMBA口服给药引起的胃、肝和肺肿瘤的发生率以及ENU治疗引起的肺肿瘤的发生率比野生型小鼠敏感得多。我们检测到<182>MT敲除小鼠和接受DMBA/TPA联合治疗的野生型小鼠乳头状瘤组织中c-Ha-ras第61密码子A^至T的颠换。MT对B[a] P诱发的微核和X射线诱发的DNA氧化损伤及微核均有明显的抑制作用。此外,MT在DMBA/TPA诱导的两阶段皮肤癌发生的起始阶段和促进阶段均具有抗肿瘤作用,提示MT在环境致癌过程中起重要的抗癌作用。
英文摘要
To elucidate the role of metallothionein (MT) in the environmental carcinogenesis, we examined the effect of MT on the two-stage skin carcinogenesis induced by 7, 12-dimethylbenz[a]anthracene (DMBA)/12-Οtetradecanoylphorbol-13-acetate (TPA), multiple organs carcinogenesis induced by DMBA oral administration and lung carcinogenesis induced by N-ethyl-N-nitrosourea (ENU) using MT-null mice whose expression of MT-I and MT-II, major isoforms of MT, were suppressed. In addition, we also eximined the susceptibility of MT-null mice in the genotoxicity caused by DMBA/TPA, benzo[a]pyren (B[a]P) or X-irradiation.MT-null mice were found to be much more sensitive than wild-type mice to incidence of skin tumor by DMBA/TPA combination, incidence of tumors in the stomach, liver and lung by DMBA oral administration and incidence of lung tumor by ENU treatment.Moreover, we detected transversion of A^<182> to T in codon 61 of c-Ha-ras in papilloma tissues of MT-null mice and wild-type mice treated with DMBA/TPA combination. It was showed using MT-null mice and wild-type mice that MT prevented B[a]P-induced micronucleus and X-irradiation-induced oxidative DNA damage and micronucleus. In addition, it was clear that MT has antitumorigenic potential in both the initiation and promotion stage of DMBA/TPA-induced two-stage skin carcinogenesis.These results suggest that MT plays an important role as anticarcinogenic factor in environmental carcinogenesis.
期刊论文(4)
专著(0)
科研奖励(0)
会议论文
Junko S.Suzuki: "Metallothionein deficiency enhances skin carcinogenesis induced by 7, 12-dimethylbenz[a]anthracene and 12-O-tetradecanoylphorbol-13-acetate in metallothionein-null mice"Carcinogenesis. (2003)
Junko S.Suzuki:“金属硫蛋白缺乏会增强金属硫蛋白缺失小鼠中 7, 12-二甲基苯并[a]蒽和 12-O-十四烷酰佛波醇-13-乙酸酯诱导的皮肤癌发生”。
DOI: --
发表时间:
期刊:
影响因子: --
作者: []
通讯作者:
Junko S. Suzuki, Noriko Nishimura, Baoxu Zhang,Yoko Nakatsuru, Shizuko Kobayashi, Masahiko Satoh and Chiharu Tohyama: "Metallothionein deficiency enhances skin carcinogenesis Induced by 7, 12-dimrthylbenz[a]anthracene and 12-Ο-tetra-Decanoylphorbol-13-ace
Junko S. Suzuki、Noriko Nishimura、Baoxu Zhu、Yoko Nakatsuru、Shizuko Kobayashi、Masahiko Satoh 和 Chiharu Tohyama:“金属硫蛋白缺乏会增强 7, 12-二甲基苯并[a]蒽和 12-Ο-四-癸酰佛醇-13 诱导的皮肤癌发生-高手
DOI: --
发表时间:
期刊:
影响因子: --
作者: []
通讯作者:
Elucidation of developmental neurotoxicity mechanisms of polycyclic aromatic hydrocarbons via aryl hydrocarbon receptor
  • 批准号:
    21390186
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
  • 资助金额:
    $11.65万
  • 财政年份:
    2009
  • 负责人:
    TOHYAMA Chiharu
  • 依托单位:
The profiling of gene expression evoked by co-exposure to dioxin and polychlorinated biphenyl.
NEW METHODOLOGY FOR EVALUATION OF RENAL DAMAGE CAUSED BY HEAVY METALS ON THE TUBULAR LEVEL
  • 批准号:
    05680471
  • 项目类别:
    Grant-in-Aid for General Scientific Research (C)
  • 资助金额:
    $1.28万
  • 财政年份:
    1993
  • 负责人:
    TOHYAMA Chiharu
  • 依托单位:
海外基金