On-site biological monitoring for exposure to VOCs by portable gas-chromatograph

挥发性有机化合物（VOCs）暴露的生物监测已通过其特定的尿液代谢产物实现。但是，已知生物流体中的母体VOC的浓度，例如。与血液和尿液一样，与其代谢物相比，一般显示出与接触强度更密切的相关性。在过去的10年中，许多出版物表明，尿液中的VOC与环境暴露有很好的相关性，因此可以用于生物监测。在尿液分析中，受试者可以很容易地提供足够体积的样本进行分析。但是，生物体液中的挥发性有机化合物容易挥发，可能会造成内容物的损失。对于生物体液中挥发性有机化合物的分析定量，样品采集和保存过程中挥发性有机化合物的损失是最重要的不确定性，在这项工作中，我们开发了一种方法，用于现场分析生物体液中挥发性有机化合物的顶空气相色谱法，因为现场分析可以最大限度地减少损失。我们应用小瓶顶空分析和注射器顶空分析，采用“旅行者”，一个现场便携式气相色谱仪配备光离子化检测器（PID）。采用该方法成功地测定了血液和尿液中痕量苯、甲苯、三氯乙烯和四氯乙烯等挥发性有机物的浓度，检测限为亚ppb级。该方法可方便地对非暴露对象的环境VOCs暴露进行现场生物监测

Biological monitoring of exposure to volatile organic compounds (VOCs) has been achieved via their specific urinary metabolites. But, it is known that concentrations of parent VOCs in biological fluids such. as blood and urine, generally show closer correlation with the exposure intensity than their metabolites. In the last 10 years many publications have shown that the urinary VOCs are well correlated with environmental exposure and could therefore be used for biological monitoring, In the urinalysis, subjects can easily provide samples of sufficient volume for analysis. But, VOCs in biological fluids can be volatilized easily and that may cause loss of contents. For analytical quantification of VOCs in biological fluids, loss of VOCs during specimen collection and preservation is the most important uncertaintiesIn this work, we developed the method far on-site analysis of VOCs in biological fluids using head space gas chromatography, as on-site analysis can minimize a loss. We applied vial head space analysis and syringe head space analysis employing "Voyager", a field portable gas-chromatograph equipped photoionization detector (PID). By proposed method, blood and urinary trace concentrations of VOCs such as benzene, toluene, trichloroethylene and tetrachloroethylene were successfully determined at sub-ppb level. On-site biological monitoring of environmental VOCs exposure of non-exposed subjects can be performed easily by the proposed method