Studies about lung metastasis and regional lymph node metastasis in SCC

FI细胞系是一种高度恶性的鳞状细胞癌，在裸鼠和患者体内具有发生肺转移、区域淋巴结转移、结外扩散、高钙血症、白细胞增多和恶病质的潜力。一只携带FI肿瘤的小鼠患上恶病质，由于其恶性，一个月内死亡。将肺转移细胞系和区域淋巴结转移细胞系分别接种于裸鼠皮下，分别建立了易发生肺转移的L细胞和易发生区域淋巴结转移的N细胞。与亲本FI细胞相比，L细胞和N细胞在形态上具有相对疏松的细胞-细胞接触。L细胞(W.B.C./体积：1×10~(-5)/cm~3)比FI细胞(5×10~(-4)/cm~3)出现更严重的白细胞增多症。L细胞条件培养液中粒细胞集落刺激因子的产生(20 ng/ml作用48h)约为…比FI细胞高50倍以上。N细胞CM产生的G-CSF(1 ng/ml，作用48h)约为FI细胞的10倍。由于FI细胞有G-CSFR的表达，用重组人粒细胞集落刺激因子和抗G-CSF中和抗体对FI细胞和L细胞增殖的实验表明，G-CSF可能是FI细胞的弱自分泌生长因子。其次，在口腔鳞癌患者中，我们研究了G-CSF和G-CSFR的表达是否与肺转移或淋巴结转移有关。结果显示：(1)106例口腔鳞状细胞癌中，G-CSFR阳性56例(53%)，G-CSF阳性56例(53%)。(2)G-CSFR的表达与肿瘤大小(P=0.0078)、疾病分期(P=0.0084)、死亡(P=0.012)及G-CSF的表达显著相关(P=0.039)。(4)与区域淋巴结转移和肺转移均无相关性。G-CSFR阳性患者的5年生存率为78%，G-CSFR阴性患者的5年生存率为46%，两者差异有统计学意义，提示G-CSFR表达与口腔鳞癌患者的预后有关。较少

A high malignant squamous cell carcinoma(SCC), FI cell line has a potential to develop lung metastasis, regional lymph nodes metastasis ; extra-nodal spreads, hypercalcemia, leukocytosis and cachexia in nude mice as well as in the patient. A mouse bearing FI tumor developed cachexia and died within a month, because of its malignancy. Repeating several procedures which the cell line derived from lung metastases, the other cell from regional lymph nodes metastases are again inoculated subcutaneously to nude mice, we established L cell and N cell which are easy to develop metastases to lung and regional lymph nodes, respectively. Compared with characteristics of parental FI cell, morphologically, L cell and N cell have relatively loose cell-cell contact. L cell (W.B.C./Volume : 1 X 10^5/cm^3) developed leukocytosis more severe than that of FI cell (5 X 10^4/cm^3). Granulocyte-Colony Stimulating Factor (G-CSF) production (20 ng/ml for 48h) in the conditioned media(CM) of L cell were about … More 50 times higher than those of FI cell. G-CSF production (1 ng/ml for 48h) in the CM of N cell were about 10 times higher than that of FI cell. As FI cell has G-CSF Receptor (G-CSFR) expression, the experiments related with FI cell and L cell proliferation used rG-CSF and anti-G-CSF neutralizing antibody, indicated that G-CSF might be weak autocrine growth factor for FI cell. Next, in patients with oral SCC we investigated whether G-CSF and G-CSFR expression were generally related with lung metastases or lymph nodes metastases or not. The results were as follows (1) Out of 106 oral SCCs, 56 cases(53 %) were positive for G-CSFR and 56 (53 %) were positive for G-CSF. (2) Expression of G-CSFR correlated significantly with Tumor size (P=0.0078), Stage of disease(P=0,0084), Death(P=0.012) and Expression of G-CSF(P=0.05), (3) Expression of G-CSF correlated significantly with Tumor size(P=0.039) and Expression of G-CSF(P=0.05). (4) None of them correlated with regional lymph nodes metastases nor lung metastases. 5-year-survival-rate was 78 % in G-CSFR-positive, 46 % in G-CSFR-negative, respectively and these are statistically significant.These results indicated that expression of G-CSFR correlates with prognosis of patients with oral SCC. Less