医薬品化学領域における生体超分子集合体の高次微細構造と機能に関する研究

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In this project, we performed several researches about the highly detailed structure and the function on a bio-supermolecular assembly which are shown in lower (1) - (5).(1) The binding study using high-performance frontal analysis found that the oxidation of low-density lipoprotein (LDL) enhances the drug binding affinity of LDL, and the increase in negative net charge due to LDL oxidation gives significant effect upon this binding enhancement. It was also found that the major genetic variants of AGP show different drug binding property, and their drug binding affinity and the enantioselectivity strongly depend upon the pH condition of sample solution.(2) Interaction and activation of plasma apolipoproteins A-1 and E at lipid particle surface were influenced by the amphipathic surface topology. Not only surface but core lipid composition of particles played roles in the topology-formation.(3) We examined the Gi activity of partial peptides of a Prostaglandin and an Opioid receptors, a … More nd the molecular interactions. All peptides formed the stable complex with Gi and activated G protein further. As a result of NMR structural analysis, the helix structure is found on the N-terminal.The potion forms a positive charge cluster and is thought that it activates G protein.(4) The effects of small peptides on the secondary structures of the peptides dissected from such amyloidgenic proteins as Aβ(Alzheimer's sisease), α-synuclein/NAC (Parkinson's disease), and PrP (prion disease) were studied. It was found that Ac-ELVFFAKK-NH_2 in Aβ(1-28), Ac-ETVK-NH_2 and Ac-KTVE-NH_2 in NAC(19-35), and Ac-EFGNK-NH_2 in and Ac-EYYEK-NH_2 in PrP(129-154) interact specifically with the peptides. Each of these small peptides could be a lead compound for designing a therapeutic agent for the diseases.(5) The statistical properties of the curved bilayer membranes of DPPC and the interactions of vitamin E with the planar membranes of DPPC in the liquid-crystal phase were studied by Monte-Calro simulation. Less