MOLECULAR BIOLOGICAL ANALYSES OF THE BEHAVIORS OF THE NEMATODE C.ELEGANS
线虫行为的分子生物学分析
基本信息
- 批准号:12480212
- 负责人:
- 金额:$ 9.15万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (B)
- 财政年份:2000
- 资助国家:日本
- 起止时间:2000 至 2003
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
The genetic basis of behavior was studied by using the nematode C.elegans. (a) Studies on synthetic dauer-constitutive mutants yielded the following results. The Tbx2 transcription factor homologue function's in olfactory adaptation in AWC sensory neurons. The Tyr-phosphatase homologue SDF-13 acts in steroid hormone metabolism in XXXL/R cells whose function had been unknown. The multidrug resistance-associated protein homologue MRP-1 plays a role in the regulation of dauer larva formation in the intestine, pharynx and nervous system, while human MRP1 can substitute for C.elegans MRP-1 in this function. (b) Class 1 flr mutations, which confer abnormality in growth, defecation and sensory functions, and class 2 flr mutations, which suppress some of the class 1 phenotypes were investigated. New class 2 mutations were isolated as suppressors serotonin-sensitivity of class 1 mutations. Class 2 mutants showed shorter longevity. (c) hen-1 mutants, which prefer avoidance of copper ion to chemotaxis to odorants, showed abnormality also in the learning of temperature and starvation. hen-1 gene functions non-cell-autonomously in two pairs of neurons at the adult stage. A deletion mutant in hen-1 showed essentially the same phenotype as the original missense allele ut236. (d) Animals showed increased chemotaxis to butanone after conditioning with food and butanone, and mutants abnormal in this behavior were isolated. One of them, ut305, was also abnormal in adaptation to benzaldehyde and isoamyl alcohol. Th ut305 gene encoded a novel membrane protein and was expressed in many pharyngeal neurons and AIA interneurons. (e) Changes of behavior after prolonged exposure to noxious stimuli were studied. On the basis of these results, it has become clear that many behaviors are regulated by signals of food and starvation through multiple pathways. This is an important clue to the central control mechanism of behavior in C.elegans.
利用线虫秀丽隐杆线虫研究了行为的遗传基础。 (a) 对合成 dauer 组成型突变体的研究产生了以下结果。 Tbx2 转录因子同源物在 AWC 感觉神经元的嗅觉适应中发挥作用。酪氨酸磷酸酶同源物 SDF-13 作用于 XXXL/R 细胞中的类固醇激素代谢,其功能尚不清楚。多药耐药相关蛋白同源物MRP-1在肠道、咽部和神经系统中对多尔幼虫的形成起到调节作用,而人MRP1可以替代线虫MRP-1的这一功能。 (b) 研究了导致生长、排便和感觉功能异常的 1 类 flr 突变和抑制某些 1 类表型的 2 类 flr 突变。新的 2 类突变被分离为 1 类突变的血清素敏感性抑制因子。 2 类突变体的寿命较短。 (c) hen-1突变体更喜欢避免铜离子而不是趋化性或气味剂,在温度和饥饿的学习中也表现出异常。 hen-1 基因在成年阶段的两对神经元中非细胞自主地发挥作用。 hen-1 中的缺失突变体表现出与原始错义等位基因 ut236 基本相同的表型。 (d)用食物和丁酮调节后,动物表现出对丁酮的趋化性增加,并且分离出这种行为异常的突变体。其中之一,ut305,对苯甲醛和异戊醇的适应也异常。 Th ut305 基因编码一种新型膜蛋白,并在许多咽神经元和 AIA 中间神经元中表达。 (e) 研究了长期暴露于有害刺激后行为的变化。根据这些结果,很明显,许多行为是通过多种途径受到食物和饥饿信号的调节。这是了解线虫行为的中央控制机制的重要线索。
项目成果
期刊论文数量(30)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Shioi et al.: "Mutations affecing nerve attachment of Caenorhabditis elegans"Genetics. 157. 1611-1622 (2001)
Shioi 等人:“影响秀丽隐杆线虫神经附着的突变”遗传学。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Aoki. et al..: "Characterization of Ce-atl-1,an ATM-like gene from Caenorhabditis elegans."Mol.Gen.Genet.. 264. 119-126 (2000)
青木。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
K.Miyahara, N.Suzuki, T.Ishiharra, E.Tsuchiya, I.Katsura: "TBX2/TBX3 transcriptional factor homologue controls olfactory adaptation in Caenorhabditis elegans."J.Neurobiol.. 58. 392-402 (2004)
K.Miyahara、N.Suzuki、T.Ishiharra、E.Tsuchiya、I.Katsura:“TBX2/TBX3 转录因子同源物控制秀丽隐杆线虫的嗅觉适应。”J.Neurobiol.. 58. 392-402 (2004)
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Kuhara et al.: "Negative regulation and gain control of sensory neurons by the C. elegans Calcineurin TAX-6"Neuron. 33. 751-763 (2002)
Kuhara 等人:“线虫钙调神经磷酸酶 TAX-6 对感觉神经元的负调控和增益控制”神经元。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Asahina et al.: "The conserved nuclear receptor Ftz-F1 is required for embryogenesis, moulting and reproduction in Caenorhabditis elegans"Genes to Cells. 5. 711-723 (2000)
Asahina 等人:“秀丽隐杆线虫的胚胎发生、蜕皮和繁殖需要保守的核受体 Ftz-F1”Genes to Cells。
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- 影响因子:0
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KATSURA Isao其他文献
KATSURA Isao的其他文献
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{{ truncateString('KATSURA Isao', 18)}}的其他基金
Molecular Biological Analysis of Behavioral Control in the Nematode C. elegans
线虫行为控制的分子生物学分析。
- 批准号:
16207012 - 财政年份:2004
- 资助金额:
$ 9.15万 - 项目类别:
Grant-in-Aid for Scientific Research (A)
Molecular Biological Analyses of the Neural Functions of the Nematode C. elegans
线虫神经功能的分子生物学分析 线虫
- 批准号:
09480190 - 财政年份:1997
- 资助金额:
$ 9.15万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Molecular Mechanisms of Differentiation and Proliferation Switches
分化和增殖开关的分子机制
- 批准号:
05269102 - 财政年份:1993
- 资助金额:
$ 9.15万 - 项目类别:
Grant-in-Aid for Scientific Research on Priority Areas
Role of Signal-transduction in the Development of C.elegans
信号转导在秀丽隐杆线虫发育中的作用
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04454615 - 财政年份:1992
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$ 9.15万 - 项目类别:
Grant-in-Aid for General Scientific Research (B)
Mechanisms of Size- and Shape-Determination in Supramolecular Structures by Genetic Information
通过遗传信息确定超分子结构的尺寸和形状的机制
- 批准号:
60580206 - 财政年份:1985
- 资助金额:
$ 9.15万 - 项目类别:
Grant-in-Aid for General Scientific Research (C)
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