Application of buoyant marine cyanobacteriaon to eco-monitoring
浮力海洋蓝藻在生态监测中的应用
基本信息
- 批准号:12555226
- 负责人:
- 金额:$ 8.26万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (B)
- 财政年份:2000
- 资助国家:日本
- 起止时间:2000 至 2002
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
This study aimed to construct a monitoring system for environmental toxic factors employing buoyant marine cyanobacteria harboring a reporter gene with environmental stimulus-response elements.Marine cyanobacteria from our culture collection were screened for buoyancy using the sucrosegradient method. Furthermore, floating culture system for marine cyanobacreria was also constructed using buoyant coal-ash materials.In order to construct recombinant cyanobacteria for environmental monitoring, we isolated stimulus-response elements (the stimulus-inducible promoter and its regulator region) from a marine cyanobacterium. Synechococcus sp. through its mini-genomic library using the shuttle vector pSRL4 with luxAB genes as a reporter. Three stimulus-response cyanobacterial clones were obtained from 181 recombinant clones where one clone for UVA responsive and two clones for NaCl. Furthermore, several strong promoters were also found. Sequencing analysis showed that these clones had transcriptional control regions and promoters.These results shows that eco-monitoring for several environmental impacts could be achieved by using above reporter cyanobacteria and floating system
本研究旨在利用携带环境刺激-反应因子报告基因的海洋浮力蓝藻构建环境毒性因子监测系统。从我们的培养收集海洋蓝藻筛选浮力使用蔗糖梯度法。此外,还以浮力粉煤灰为原料,构建了海洋蓝藻浮力培养体系。为了构建用于环境监测的重组蓝藻,我们从海洋蓝藻中分离出刺激-响应元件(刺激诱导启动子及其调控区)。利用带luxAB基因的穿梭载体pSRL4作为报告基因,通过其微型基因组文库进行了研究。从181个重组克隆中获得3个刺激反应蓝藻克隆,其中1个对UVA反应,2个对NaCl反应。此外,还发现了几个强启动子。测序分析表明,这些克隆具有转录控制区和启动子。这些结果表明,利用上述报告蓝藻和漂浮系统可以实现多种环境影响的生态监测
项目成果
期刊论文数量(62)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
H.Takeyama, M.Wada, T.Matsunaga: "Screening of soil bacteria for production of bio-cleaner"Appl.Biochem.Biotechnol. 98/100. 319-326 (2002)
H.Takeyama、M.Wada、T.Matsunaga:“筛选用于生产生物清洁剂的土壤细菌”Appl.Biochem.Biotechnol。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Mitsufumi Matsumoto: "Floating cultivation of marine cyanobacteria using coal fly ash."Appl.Biochem.Biotechnol.. 84/86. 51-57 (2000)
Mitsufumi Matsumoto:“利用煤粉灰漂浮培养海洋蓝藻。”Appl.Biochem.Biotechnol.. 84/86。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
M. Matsumoto, H. Miyashita, H. Takeyama, T. Matsunaga: "Screening of buoyant marine cyanobacteria using sucrose gradient centrifugation"J. Appl. Phycol.. 14. 91-95 (2002)
M. Matsumoto,H. Miyashita,H. Takeyama,T. Matsunaga:“利用蔗糖梯度离心筛选浮力海洋蓝藻”J。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Haruko Takeyama: "Screening of soil bacteria for production of bio-cleaner"Appl. Biochem. Biotechnol.. (in press).
Haruko Takeyama:“筛选用于生产生物清洁剂的土壤细菌”Appl。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
R.Yu, A.Yamada, K.Watanabe, K.Yazawa, H.Takeyama, T.Matsunaga, R.Kurane: "Production of eicosapentaenoic acid by a recombinant marine cyanobacterium, Synechococcus sp."Lipids. 35. 1061-1064 (2000)
R.Yu、A.Yamada、K.Watanabe、K.Yazawa、H.Takeyama、T.Matsunaga、R.Kurane:“重组海洋蓝藻聚球藻生产二十碳五烯酸”脂质。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
TAKEYAMA Haruko其他文献
Droplet microfluidics toward accurate genome sequencing of environmental bacteria at the single-cell level
液滴微流体技术可在单细胞水平上对环境细菌进行准确的基因组测序
- DOI:
- 发表时间:
2018 - 期刊:
- 影响因子:0
- 作者:
NISHIKAWA Yohei;HOSOKAWA Masahito;KOGAWA Masato;TAKAHASHI Kai;TAKEYAMA Haruko - 通讯作者:
TAKEYAMA Haruko
TAKEYAMA Haruko的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('TAKEYAMA Haruko', 18)}}的其他基金
Development of technique for digital counting of DNA copy number at the single-cell level
单细胞水平DNA拷贝数数字计数技术的开发
- 批准号:
24360344 - 财政年份:2012
- 资助金额:
$ 8.26万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Development of a pyrosequence-based technique for HIV drug-resistance diagnosis
开发基于焦磷酸序列的 HIV 耐药性诊断技术
- 批准号:
21360408 - 财政年份:2009
- 资助金额:
$ 8.26万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Nano-screening of marine symbiotic consortium for useful bio-materials and their analyses
海洋共生体有用生物材料的纳米筛选及其分析
- 批准号:
15360437 - 财政年份:2003
- 资助金额:
$ 8.26万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Screening of enviromental stress response elements and their application
环境应激反应元件筛选及其应用
- 批准号:
11650813 - 财政年份:1999
- 资助金额:
$ 8.26万 - 项目类别:
Grant-in-Aid for Scientific Research (C)