Application of buoyant marine cyanobacteriaon to eco-monitoring

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This study aimed to construct a monitoring system for environmental toxic factors employing buoyant marine cyanobacteria harboring a reporter gene with environmental stimulus-response elements.Marine cyanobacteria from our culture collection were screened for buoyancy using the sucrosegradient method. Furthermore, floating culture system for marine cyanobacreria was also constructed using buoyant coal-ash materials.In order to construct recombinant cyanobacteria for environmental monitoring, we isolated stimulus-response elements (the stimulus-inducible promoter and its regulator region) from a marine cyanobacterium. Synechococcus sp. through its mini-genomic library using the shuttle vector pSRL4 with luxAB genes as a reporter. Three stimulus-response cyanobacterial clones were obtained from 181 recombinant clones where one clone for UVA responsive and two clones for NaCl. Furthermore, several strong promoters were also found. Sequencing analysis showed that these clones had transcriptional control regions and promoters.These results shows that eco-monitoring for several environmental impacts could be achieved by using above reporter cyanobacteria and floating system