Development of a pyrosequence-based technique for HIV drug-resistance diagnosis

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The aim of this research is to develop a single-cell analytical technique based on pyrosequencing to accurately identify diverse mutations within HIV and to conduct sequence-based elucidation of the mechanisms related to HIV quasispecies formation. As a result, we were successful in the establishment of a system for measuring cDNA molecules to as low as 10 copies. Furthermore, we were also successful in tracing the coevolution between the subtypes of HIV quasispecies-related proviral DNA, Gag and PR.