Study on Development of Second Harmonic Generation Microscopy (SHGM)

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The first object of this project was to develop a Second Harmonic Generation Microscopy (SHGM) using a strong pulse YAG laser. This is to apply for study of monolayers at the water surface, solution surfaces or at the transparent solid surfaces. The YAG laser and an optical zooming microscope were set on the arms of a goniometer. The incident angle of the p-polarized laser light can be adjusted to the Brewster angle of the transparent medium to avoid exposure the CCD camera of higher sensitivity to the strong reflected light. We could observe SHG images from some samples on transparent supporting medium. Beside of the development of the SHGM, we proceeded studies on the structures of Langmuir and Gibbs monolayers using vibrational spectroscopy and Atomic Force Microscopy (AFM).