权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Development of bioremediation processes under anoxic conditions using denitrifying bacteria

使用反硝化细菌开发缺氧条件下的生物修复工艺

基本信息

批准号：
12556014
负责人：
KATO Nobuo
金额：
$ 8.19万
依托单位：
KYOTO UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2002
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-12556014/
关键词：
Azoarcus sp Thauera sp Magnetospirillum sp denitrifying bacteria toluene anaerobic degradation of toluene benzyl succinate synthase benzoyl CoA reductase 脱窒性細菌嫌気分解バイオレメディエーション Thauera sp.Mognetospirillum sp.Thauera sp. DNT1 /

项目摘要

1) Analysis of microbial consortia for degradation of aromatic compounds under denitrifying conditionsBacteria that degrade aromatic compounds under denitrifying conditions had been restricted strains belonging to genera Thauera and Azoarcus. In this project, we have isolated a number of novel bacterial strains that belonged to genus Magnetospirillum. The isolates were divided into 3 groups through phylogenetic analysis with 16S rDNA, which correspond to availability of aromatic compounds for growth.2) Pathway for degradation of aromatic compounds by the isolates(1) Toluene degradation by Thauera sp. DNT-1: The organism was found to be able to degrade toluene under both aerobic and denitrifying conditions. Toluene dioxygenase (Tod) and benzyl succinate synthase (Bss) were cloned. From the results of transcriptional analyses of the genes, Bss could be effective only under anaerobic conditions, while tod gene product functioned under aerobic conditions.(2) Cloning of genes for toluene degradation by Magnetospirillum spp. The genes encoding main enzymes participating in toluene degradation under denitrifying conditions were cloned from Magnetospirillum sp. TS-6. From the result, the degradation pathway of the organism could be confirmed.3) Assignment of the denitrifying bacteria under in-situ-bioremediationBenzoyl CoA reductase was found as a key enzyme for the ring fission a variety of anaerobic toluenedegraders. We proposed a monitoring system for anaerobic aromatic-compound-degraders using benzoyl CoA reductase genes as the probe.

1)反硝化条件下降解芳香族化合物的微生物群落分析在反硝化条件下降解芳香族化合物的细菌为限制菌株，分属于梭菌属和固氮菌属。在本项目中，我们分离到了一批属于磁螺菌属的新细菌菌株。通过16S rDNA系统发育分析，将这些菌株分为3个类群，分别与芳香族化合物的生长有效性相对应。2)菌株降解芳香族化合物的途径(1)塔伊拉细菌降解甲苯。DNT-1：发现该菌在好氧和反硝化条件下都能降解甲苯。克隆了甲苯双加氧酶(TOD)和琥珀酸苄酯合成酶(BSS)。从基因转录分析的结果来看，BSS只有在厌氧条件下才能发挥作用，而TOD基因产物在有氧条件下才能发挥作用。(2)磁螺杆菌甲苯降解相关基因的克隆。从磁螺菌中克隆了参与反硝化条件下甲苯降解的主要酶基因。TS-6。3)原位生物修复条件下反硝化细菌的归属研究发现，苯甲酰辅酶A还原酶是多种厌氧甲苯降解菌环裂的关键酶。我们提出了一种以苯甲酰辅酶A还原酶基因为探针的厌氧芳香族化合物降解菌监测系统。