Molecular mechanisms of synaptic plasticity : specific involvement of various aspects of calcium dependent processes

突触可塑性的分子机制：钙依赖性过程各个方面的具体参与

• 批准号: 09480229
• 负责人: KATO Nobuo
• 金额: $ 7.23万
• 依托单位: KYOTO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B).
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-09480229/
• 关键词: Long-term potentiation; Long-term depression; Synaptic plasticity; calcium release; シナプス伝達; カルシウム流入; 細胞内小胞体; カルシウムイメージング; カルシウム; 大脳皮質; 海馬; 活動電位; シナプス; カルシウムチャンネル; 神経可塑性; 細胞内カルシウム; カルシウム測光

In neurons and non-excitable cells alike, single intracellular signaling molecules seem to be involved in more than one signaling cascade, so as to mediate more than one intracellular messages. How the same signal molecule tells two or more biologically different contents of intracellular messages is puzzling. One fruitful approach to address this puzzle would be to study the mechanism of bi-directional regulation of synaptic plasticity in neurons, which is dependent on the calcium ion. Long-term potentiation (LTP) and depression (LTD), which represent up- and down-regulations of synaptic efficiency respectively, are widely accepted models for studying molecular mechanisms of memory. Increases of intracellular Ca2+ concentration are required for induction of both LTP and LTD.These two types of synaptic regulations are shown to be discriminately induced, depending on the same intracellular Ca2+ ions. The findings obtained in the present project suggest that intracellular Ca2+ can carry … More sufficient information to initiate discriminative induction of LTP or LTD.The present project discovered a novel form of calcium release, which is caused by a synergistic activation of the IP_3 receptors on the endoplasmic reticulum by its two ligands calcium and IP_3. This novel calcium release was further demonstrated to correlate with an activity-dependent, negative feedback regulation of action potential firing. These findings seem to have a number of implications on neocortical physiology. First, a novel anchor molecule might exist which links IP3 receptors and voltage-dependent calcium channels. Second, the present novel calcium release may provide a site of interaction between the "slow", neuromodulator-dependent cortical projection system and the "fast" system, which most directly affects intracellular calcium concentration. Third, this novel calcium release may prevent excitotoxic neuronal death without preventing basic neuronal activity, and could therefore be therapeutically useful. The last possibility was experimentally supported in the present study. Less

在神经元和非兴奋性细胞中，单个细胞内信号分子似乎参与了多个信号级联，从而介导了多个细胞内信息。同样的信号分子如何告诉两个或更多生物上不同的细胞内信息内容令人费解。解决这一难题的一个有效的方法是研究神经元突触可塑性的双向调节机制，这依赖于钙离子。长时程增强(LTP)和长时程增强(LTD)分别代表突触效率的上调和下调，是目前广泛接受的研究记忆分子机制的模型。LTP和LTD的诱导都需要细胞内钙离子浓度的增加，这两种类型的突触调节被证明是有区别地诱导的，取决于相同的细胞内钙离子。本项目的研究结果表明，细胞内的钙离子可以携带…本项目发现了一种新的钙释放形式，它是由内质网上的IP_3受体被其两个配体钙和IP_3协同激活而引起的。这种新的钙释放被进一步证明与动作电位放电的活动依赖的负反馈调节有关。这些发现似乎对新皮质生理学有一些启示。首先，可能存在一种连接IP3受体和电压依赖性钙通道的新锚定分子。第二，目前新的钙释放机制可能提供了一个“慢”的、依赖神经调节剂的皮质投射系统和“快”系统相互作用的场所，而“快”系统最直接地影响细胞内的钙浓度。第三，这种新的钙释放可以在不阻止神经元基本活动的情况下防止兴奋性毒性神经元的死亡，因此可能在治疗上有用。最后一种可能性在本研究中得到了实验支持。较少

项目成果

Yamamoto K,Hashimoto K,Isomura Y.,Kato N: "An IP_3-assisted form of Ca^<2+>-induced Ca^<2+> release in neocortical neurons"NeuroReport. 11. 535-539 (2000)

Yamamoto K、Hashimoto K、Isomura Y.、Kato N：“新皮质神经元中 Ca^<2> 诱导的 Ca^<2> 释放的 IP_3 辅助形式”NeuroReport。

Isomura Y, Kato N: "Correlation of susceptibility to long-term ptentiation and action potential-induced calcium increases in proximal apical dendrites of developing hippocampal CA1 pyramidal cells"J Neurophysiol. 82. 1993-1999 (1999)

Isomura Y、Kato N：“发育中的海马 CA1 锥体细胞近端顶端树突中长期电位敏感性和动作电位诱导的钙增加的相关性”《神经生理学杂志》。

Yoshimura,H.,Kato,N.: "Diverse roles of intracellular cAMP in early synoptic modifications in the rat visual Cortex"J.Physiol.(London). 522. 417-426 (2000)

Yoshimura,H.,Kato,N.：“细胞内 cAMP 在大鼠视觉皮层早期天气变化中的多种作用”J.Physiol.（伦敦）。

Kato N, Isomura Y, Tanaka T: "Intracellular calcium releases facilitate induction of long-term depression."Neuropharmacology. 39. 1107-1110 (2000)

Kato N、Isomura Y、Tanaka T：“细胞内钙释放有助于诱导长期抑郁。”神经药理学。

Y.Isomura,N.Kato: "A possible regularory role of dendritic spikes in induction of long-termpotentiation at hippocampal Schaffer collateral-Ca1 synopsis"Brain Res. 883. 119-124 (2000)

Y.Isomura，N.Kato：“树突尖峰在海马 Schaffer 侧支 Ca1 长期增强诱导中的可能规律作用”Brain Res。