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In vitro model of the blood-brain barrier

血脑屏障的体外模型

基本信息

批准号：
12557009
负责人：
NIWA Masami
金额：
$ 4.86万
依托单位：
NAGASAKI UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2001
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-12557009/
关键词：
Blood-Brain Barrier astrocytes Drug-transport to brain pericytes brain capillary endothelial cell in vitro model of the BBB anti-dementia drug BBBキット

项目摘要

The blood-brain barrier (BBS) is composed of endothelial cells, pericytes and astrocytes. Ordinary, astrocyte induce strong barrier function of endothelial monolayer. But the effect of astroycte has been still unknown under pericyte influence. We made a new model of the BBB with isolated rat brain endothelial cells, pericytes and astroyctes and checked barrier function of each model. Three different models (A) only endothelial cells, B) with pericytes non-contact, C) with pericytes contact and D) with pericytes and astrocytes) were made 」>y culturing these cells on the Transwell two chamber system and their barrier functions were evaluated with permeability studies. Although the stronger barrier function was observed in an endothelial/pericytic contact model, astrocyte reduced the elevated barrier function of the co-culture model. Each three kinds of cells composing brain microvasculature have their own effects for the BBB and the interaction of all cells may be necessary for induction or maintenace of a functional BBB.

血脑屏障（BBS）由内皮细胞、周细胞和星形胶质细胞组成。通常，星形胶质细胞诱导内皮单层的强大屏障功能。但星形细胞在周细胞影响下的作用仍不清楚。我们用分离的大鼠脑内皮细胞、周细胞和星形细胞制作了新的血脑屏障模型，并检查了每个模型的屏障功能。制作了三种不同的模型（A）仅内皮细胞，B）周细胞非接触，C）周细胞接触和D）周细胞和星形胶质细胞）>在Transwell两室系统上培养这些细胞，并通过渗透性研究评估它们的屏障功能。尽管在内皮/周细胞接触模型中观察到更强的屏障功能，但星形胶质细胞降低了共培养模型中升高的屏障功能。组成脑微脉管系统的每三种细胞对 BBB 都有各自的影响，所有细胞的相互作用可能是诱导或维持功能性 BBB 所必需的。

项目成果

期刊论文数量（60）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Bela Kis, Maria A. Deli, Hideyuki Kobayashi, Csongor S. Abraham, Toshihiko, Yanagita, Hiroyuki Kaiya, Toyoshi Isse, Rieko Nishi, Sadao Gotoh, Kenji Kangawa, Akihiko Wada, John Greewood, Masami Niwa, Hiroshi Yamashita, Youichi Ueta: "adrenomedulin regulate

Bela Kis、Maria A. Deli、Hideyuki Kobayashi、Csongor S. Abraham、Toshihiko、Yanagita、Hiroyuki Kaiya、Toyoshi Isse、Rieko Nishi、Sadao Gotoh、Kenji Kangawa、Akihiko Wada、John Greewood、Masami Niwa、Hiroshi Yamashita、Youichi Ueta：