In vitro blood-brain barrier reconstruction model (BBB Kit) and their application to the functional analysis

体外血脑屏障重建模型（BBB Kit）及其在功能分析中的应用

• 批准号: 22590243
• 负责人: NIWA Masami
• 金额: $ 2.91万
• 依托单位: Nagasaki University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22590243/
• 关键词: 中枢; 末梢神経; 血液脳関門; サル血液脳関門; 血液脳関門再構成モデル; サル脳毛細血管内皮細胞; 脳ペリサイト; アストロサイト; 薬物脳内移行性; タイトジャンクション; ラット血液脳関門

The blood-brain barrier (BBB), a member of the neurovascular unit (NVU), functions in communication between brain capillary endothelial cells and other neighboring cells such as astrocytes and pericytes. Many drugs that are currently under development are intended to act centrally, but they do not cross the BBB. Furthermore, the crosstalk between the BBB and related cells contributing to the NVU remains to be elucidated. Based on our breakthrough in using rat primary brain capillary endothelial cells, pericytes and astrocytes to create a triple co-culture BBB model (BBB Kit), an in vitro models for screening the BBB penetration of drug candidates and research on BBB pathophysiology, we succeeded in modeling the monkey BBB with primary monkey brain capillary endothelial cells, rat pericytes and rat astrocytes. In addition, we constructed a porcine BBB model consisting of porcine brain capillary endothelial cells, rat pericytes, and rat astrocytes

血脑屏障（BBB）是神经血管单位（NVU）的一员，在脑毛细血管内皮细胞与其他相邻细胞（如星形胶质细胞和周细胞）之间的通讯中发挥作用。目前正在开发的许多药物旨在集中发挥作用，但它们不会穿过BBB。此外，BBB与促成NVU的相关细胞之间的串扰仍有待阐明。本课题组在利用大鼠原代脑毛细血管内皮细胞、周细胞和星形胶质细胞构建三重共培养血脑屏障模型（BBB Kit）、筛选药物体外穿透血脑屏障模型和研究血脑屏障病理生理的基础上，成功地建立了猴原代脑毛细血管内皮细胞、周细胞和星形胶质细胞的血脑屏障模型。此外，我们构建了由猪脑毛细血管内皮细胞、大鼠周细胞和大鼠星形胶质细胞组成的猪脑血脑屏障模型

项目成果

Lercanidipine Rescues Hippocampus Pyramidal Neurons from Mild Ischemia-Induced Delayed Neuronal Death in SHRSP

• DOI: 10.1007/s10571-011-9649-6
• 发表时间: 2011-01
• 期刊: Cellular and Molecular Neurobiology
• 影响因子: 4
• 作者: Y. Sakurai‐Yamashita;N. Harada;M. Niwa

An In vitro monkey BBB model.

体外猴 BBB 模型。

• 发表时间: 2010
• 作者: SNakagawa;MA Deli1;DHD Thuy;M Sagara;N Yamada;G So;R Tatsumi;K Tanaka;M Niwa
• 通讯作者: M Niwa

Protection of the Blood-Brain Barrier by Pentosan Against Amyloid-β-Induced Toxicity

• DOI: 10.3233/jad-2010-100759
• 发表时间: 2010-01-01
• 期刊: JOURNAL OF ALZHEIMERS DISEASE
• 影响因子: 4
• 作者: Deli, Maria A.;Veszelka, Szilvia;Niwa, Masami
• 通讯作者: Niwa, Masami

In vitro 血液脳関門再構成モデル(BBBキット)を用いたBBB機能の解析

使用体外血脑屏障重建模型分析 BBB 功能（BBB 试剂盒）

• 发表时间: 2012
• 作者: 中川慎介;丹羽正美
• 通讯作者: 丹羽正美

Development of in vitro monkey BBB model

猴体外BBB模型的建立

• 发表时间: 2010
• 作者: S Nakagawa;MA Deli1;DHD THUY;M Sagara;N Yamada;K Tanaka;M Niwa.
• 通讯作者: M Niwa.