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Diagnostic System for Biological Characteristics of Cancer by Genome Microarray

基因组微阵列癌症生物学特征诊断系统

基本信息

批准号：
12557019
负责人：
SASAKI Kohsuke
金额：
$ 6.91万
依托单位：
YAMAGUCHI UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2001
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-12557019/
关键词：
genome DNA genes microarray CGH BAC cancer carcinogenesis 染色体がん遺伝子

项目摘要

It is widely accepted that the development of cancer results from the accumulation of gene aberrations in cellular nucleus. In order to understand the biological characteristics of tumors and to perform the effective treatment for tumors, it is necessary to make a comprehensive analysis of genomic changes in tumor cells. Cancer shows great variation in biological behavior (the degree of malignancy) as known as tumor heterogeneity. At present, however, there are no reliable methods to estimate biological characteristics of tumor. In this project, we made basic study for developing a genome microarray system to detect aberrations in genome of tumor cells.1. The increase in DNA copy number was examined by DNA genome microarray spotted with 57 oncogenes in hepatocellular carcinoma and esophageal squamous cell carcinoma. These data have been publised in scientific journal or a paper was prepared for publication.2. We are trying to develop software for analysis of data obtained from genome microarray.3. The identification of genes involved in carcinogenesis is being tried by data extraction from comparative genomic hybridization analysis for developing genome microarray. Indeed, BAC contig have set for the interested chromosomal loci and genome array is being prepared.

人们普遍认为癌症的发生是细胞核中基因畸变积累的结果。为了了解肿瘤的生物学特性并对肿瘤进行有效的治疗，需要对肿瘤细胞的基因组变化进行全面分析。癌症在生物学行为（恶性程度）方面表现出巨大的差异，称为肿瘤异质性。然而，目前尚无可靠的方法来估计肿瘤的生物学特性。在本项目中，我们为开发检测肿瘤细胞基因组畸变的基因组微阵列系统进行了基础研究。 1．通过在肝细胞癌和食管鳞状细胞癌中点样有 57 个癌基因的 DNA 基因组微阵列来检查 DNA 拷贝数的增加。这些数据已发表在科学期刊上或准备发表论文。2．我们正在尝试开发用于分析从基因组微阵列获得的数据的软件。3．正在尝试通过从比较基因组杂交分析中提取数据来鉴定与癌发生有关的基因，以开发基因组微阵列。事实上，BAC 重叠群已针对感兴趣的染色体位点进行设置，并且正在准备基因组阵列。