The prediction of periodontal condition by the measurement of the antibody titers directed to heat shock proteins

本研究的目的是探讨牙周疾病和分泌型免疫球蛋白A（S-IgA）针对热休克蛋白（HSP）的牙周致病菌的抗体滴度之间的关系。每名参与者，转介到预防牙科诊所，德岛大学牙科医院，签署了唾液收集的知情同意书。通过对HSP 60家族5种蛋白的S-IgA免疫印迹分析，发现牙周炎患者唾液中直弯曲杆菌GroEL和牙龈卟啉单胞菌GroEL的免疫反应阳性率显著高于健康人和轻度牙龈炎患者（p<0.05）。当BIAcore 1000用于测量针对C. r的S-IgA滴度时，在牙周炎患者的唾液中观察到高水平的RU值（p<0.01）。GroEL。此外，具有深袋深度（[大于或等于]5 mm）的牙齿的百分比显示与RU值的高相关系数（r=0.50，p<0.01）。产生GroEL是为了使用如上所述的BIAcore装置建立方法，其测定仅需要约20分钟。通过对C.r. groEL基因的核苷酸序列为1641 bp，编码547个氨基酸。这些结果表明，HSP的S-IgA滴度测定可作为牙周病的预测方法，特别是作为使用BIAcore装置的快速测定方法。

The aim of this study was to investigate the relationships between periodontal conditions and the antibody titers of the secretory immunoglobulin A (S-IgA) directed to heat shock proteins (HSP) from periodontopathogenic bacteria. Each participant, referred to the Clinic of the Preventive Dentistry, The University of Tokushima Dental Hospital, signed an informed consent document for salivary collection. By Western immunoblotting analysis of S-IgA directed to 5 kinds of protein belonging to HSP 60 family, immunoreactivities of Campylobacter rectus GroEL and Porphyromonas gingivalis GroEL were detected significantly (p<0.05) in saliva from patients with periodontitis comparing to patients with healthy/slight gingivitis. High level of RU value was observed in saliva from patients with periodontitis (p<0.01) when BIAcore 1000 was used for the measurement of S-IgA titers directed to C.r. GroEL. Furthermore, percentage of teeth with deep pocket depth (【greater than or equal】5 mm) showed high correlation coefficients with RU value (r=0.50, p<0.01).The recombinant C.r. GroEL was produced in order to establish the method using BIAcore apparatus as described above, which it takes only about 20 min for an assay. By the analysis of C.r. groEL gene, it revealed that the nucleotide sequence of 1641 bp coded for a 547 amino acid protein. This result was used for the production procedure, and the recombinant protein was obtained.These results indicate that the measurement of S-IgA titers directed to HSP might be considered to be an assay for the prediction of periodontal condition, especially, to be as a rapid assay method using BIAcore apparatus.