The prediction of periodontal condition by the measurement of the antibody titers directed to heat shock proteins

通过测量热休克蛋白抗体滴度来预测牙周状况

• 批准号: 12557184
• 负责人: HINODE Daisuke
• 金额: $ 3.14万
• 依托单位: The University of Tokushima
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-12557184/
• 关键词: Heat shock proteins; Campylobacter rectus GroEL; Measurement of antibody titers; Secretory immunoglobulin A; Periodontal conditions; Gene analysis; Recombinant protein; Rapid assay method; 唾液分布型IgA; 歯周病; 歯周ポケット; BIAcore装置; リコンビナント蛋白; ウェスタンイムノ

The aim of this study was to investigate the relationships between periodontal conditions and the antibody titers of the secretory immunoglobulin A (S-IgA) directed to heat shock proteins (HSP) from periodontopathogenic bacteria. Each participant, referred to the Clinic of the Preventive Dentistry, The University of Tokushima Dental Hospital, signed an informed consent document for salivary collection. By Western immunoblotting analysis of S-IgA directed to 5 kinds of protein belonging to HSP 60 family, immunoreactivities of Campylobacter rectus GroEL and Porphyromonas gingivalis GroEL were detected significantly (p<0.05) in saliva from patients with periodontitis comparing to patients with healthy/slight gingivitis. High level of RU value was observed in saliva from patients with periodontitis (p<0.01) when BIAcore 1000 was used for the measurement of S-IgA titers directed to C.r. GroEL. Furthermore, percentage of teeth with deep pocket depth (【greater than or equal】5 mm) showed high correlation coefficients with RU value (r=0.50, p<0.01).The recombinant C.r. GroEL was produced in order to establish the method using BIAcore apparatus as described above, which it takes only about 20 min for an assay. By the analysis of C.r. groEL gene, it revealed that the nucleotide sequence of 1641 bp coded for a 547 amino acid protein. This result was used for the production procedure, and the recombinant protein was obtained.These results indicate that the measurement of S-IgA titers directed to HSP might be considered to be an assay for the prediction of periodontal condition, especially, to be as a rapid assay method using BIAcore apparatus.

本研究的目的是探讨牙周疾病与针对牙周病原菌热休克蛋白的分泌型免疫球蛋白A抗体效价的关系。每个参与者都与德岛大学牙科医院的预防牙科诊所签署了一份唾液采集的知情同意文件。通过针对HSP60家族5种蛋白的S-IgA的免疫印迹分析，发现牙周炎患者唾液中的直弯杆菌GroEL和牙龈卟啉单胞菌GroEL的免疫反应性显著高于健康/轻度牙周炎患者(P&lt；0.05)。用Biacore 1000测定慢性牙周炎患者唾液中S-IgA滴度时，发现唾液中RU值较高。格罗尔。此外，深袋深度(大于或等于)5 mm的牙齿百分比与RU值呈高度相关(r=0.50，p&lt；0.01)。为了使用如上所述的Biacore仪器建立该方法，生产了GroEL，其分析只需要大约20分钟。通过对C.R.的分析。GroEL基因全长1641bp，编码547个氨基酸。这些结果表明，针对热休克蛋白的S-Ig A滴度的测定可以被认为是一种预测牙周状况的方法，特别是作为一种使用Biacore仪器的快速检测方法。

项目成果

Hinode, D., 7 others: "Antigenic properties of the GroEL-like protein of Campylobacter rectus"Oral Microbiol.Immunol.. 17. 16-21 (2002)

Hinode, D.，其他 7 人：“直肠弯曲杆菌 GroEL 样蛋白的抗原特性”口腔微生物.免疫学.. 17. 16-21 (2002)

Yokoyama, M. et al.: "Antigenic characterization of the surface protein from Campylobacter rectus."J. Dent. Health. 50. 594-595 (2000)

Yokoyama, M. 等人：“直肠弯曲杆菌表面蛋白的抗原特征。”J.

Tanabe, S., et al.: "Helicobacter pylori and. Campylobacter rectus share a common antigen."Oral Microbiol. Immun.. 18 (in press). (2003)

Tanabe, S. 等人：“幽门螺杆菌和直肠弯曲杆菌具有共同的抗原。”口腔微生物。

Tanabe, S., 4 others: "Immunological relationship between periodontopathogenic bacteria and Helicobacter pylori"J. Dent. Res.. 80(SI). 679 (2001)

Tanabe，S.，其他 4 人：“牙周致病菌与幽门螺杆菌之间的免疫学关系”J。

赤木毅 他6名: "Campylobacter rectusとHelicobacter pyloriの共通抗原"口腔衛生学会雑誌. 50. 592-593 (2000)

Takeshi Akagi 等 6 人：“直肠弯曲杆菌和幽门螺杆菌的常见抗原”口腔健康学会杂志 50. 592-593 (2000)。