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Studies on the practical use of soybean peptides for oral health promotion in the elderly

大豆肽促进老年人口腔健康的实际应用研究

基本信息

批准号：
17592182
负责人：
HINODE Daisuke
金额：
$ 2.24万
依托单位：
The University of Tokushima
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2005
资助国家：
日本
起止时间：
2005 至 2006
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17592182/
关键词：
soybean peptide opportunistic infections Aggregatibacter actinomycetemcomitans Candida glabrata growth inhibition saliva the elderly requiring care substance P Candida glabrata 生育阻害実験 Actinobacillus actinomycetemcomitans Porphyromona

项目摘要

In order to investigate the hypothesis that dietary consumption of soybean peptides may be beneficial for oral health promotion in the elderly, we studied their effects on growth of oral pathogenic microorganisms. The soybean peptides "Hinute-R" (average chain length 3-4) inhibited the growth of Aggregatibacter actinomycetemcomitans, an etiologic agent of periodontitis. It also inhibited the growth in early stage of Candida albicans and Candida glabrata, which can cause opportunistic oral infections. Two fractions were obtained from Hinute-R by gel filtration chromatography. The fraction I, showing a molecular weight of approximately 10,000 daltons, was remarkably effective in inhibiting the growth of C. glabrata when compared to Hinute-R and fraction II having a molecular weight of approximately 2,000 daltons. The presence of LLPHH, anti-oxidative peptide isolated from digests of a soybean protein, at 2.5 and 12.5 mg/ml reduced the growth of C. glabrata by 23% and 42%, respectively. No toxicity of soybean peptides was observed against human cells. These results support the potential of soybean peptides for elderly subjects with poor resistance to infections.In the second part of the project, we showed that the suction method is useful to obtain information regarding the salivary protein profiles in aged subjects who cannot eat or spit saliva easily. As preliminary studies for our clinical trials, we investigated the relationship between salivary components and oral conditions in the elderly requiring care. Among components investigated, the substance P, an endogenous substance causing cough reflex and involved in swallowing function, was positively correlatedwith the number of teeth present. This information may be useful for further clinical trials using the soybean peptides.

为了研究饮食中摄入大豆肽可能有益于促进老年人口腔健康的假设，我们研究了它们对口腔病原微生物生长的影响。大豆肽“Hinute-R”（平均链长3-4）可抑制牙周炎病原体Aggregatibacter actinomycetemcomitans的生长。它还可以抑制白色念珠菌和光滑念珠菌的早期生长，从而引起机会性口腔感染。通过凝胶过滤色谱法从 Hinate-R 获得两个级分。当与Hinute-R和具有大约2,000道尔顿分子量的级分II相比时，显示大约10,000道尔顿分子量的级分I在抑制光滑念珠菌生长方面非常有效。 LLPHH（一种从大豆蛋白消化物中分离出来的抗氧化肽）的存在，浓度为 2.5 和 12.5 mg/ml 时，光滑念珠菌的生长分别减少了 23% 和 42%。未观察到大豆肽对人体细胞有毒性。这些结果支持了大豆肽对于抵抗感染能力差的老年受试者的潜力。在该项目的第二部分中，我们表明，抽吸方法可用于获取有关不能轻易进食或吐唾液的老年受试者的唾液蛋白质谱的信息。作为临床试验的初步研究，我们调查了需要护理的老年人的唾液成分与口腔状况之间的关系。在研究的成分中，P物质是一种引起咳嗽反射并参与吞咽功能的内源性物质，与牙齿的数量呈正相关。该信息可能对使用大豆肽进行进一步的临床试验有用。