Investigation and biochemical study of novel neurotoxins and thrombo-toxin from the venoms of Elapidae and Viperidae snakes.

蛇毒含有多种有趣的蛋白质，包括促凝剂、抗凝剂、纤溶成分、血小板激动剂、血小板拮抗剂和出血成分。蛇毒的功能成分之一是C型凝集素样蛋白(CLP)家族的成员之一，该家族包括凝血因子IX/X结合蛋白(IX-X-BP)、钙依赖凝血酶原激活剂(Carin激活酶-1)、RVV-X(罗素蛇毒的凝血因子X激活剂)、白聚集素-B(血小板激动剂)和黄素-A(血小板拮抗剂)。这些CLP执行各种活动，并且已经对各种CLP进行了排序。我们从东方棉口蛇(Agkislrodon Pisciorus Pisciorus)、眼镜王蛇(Ophiphagus Hannah)和西部响尾蛇(Crotalus Atrox)的毒液中分离、鉴定和克隆了三个新的脆性家族蛋白基因(Pisciorin、ophanin和Catrin)。我们的结果表明，蛇毒脆性家族蛋白在不同大陆的蛇科和眼镜蛇科中分布广泛，表明脆性家族蛋白构成了一组新的蛇毒蛋白。

Snake venom contains a diverse range of interesting proteins, including procoagulants, anticoagulants, fibrinolytic components, platelet agonists, platelet antagonists, and hemorrhagic components. One of the functional components of snake venom is a member of the C-type lectin-like protein (CLP) family that includes factor IX/X-binding protein (IX-X-bp), carinactivase-1 (calcium-dependent prothrombin activator), RVV-X (factor X activator of Russell's viper venom), alboaggregin-B (platelet agonist), and flavocetin-A (platelet antagonist). These CLPs perform a variety of activities, and various CLPs have been sequenced. CLPs have proved to be useful tools in elucidating the complex mechanisms involved in clotting and platelet formation and the structure-function relationship of human clotting factors and glycoproteins of platelets.We isolated, characterized, and cloned genes for three novel CRISP family proteins (piscivorin, ophanin, and catrin) from the venom of eastern cottonmouth (Agkislrodon piscivorus piscivorus), king cobra (Ophiophagus hannah), and western diamondback rattlesnake (Crotalus atrox). Our results show the wide distribution of snake venom CRISP family proteins among Viperidae and Elapidae from different continents, indicating that CRISP family proteins compose a new group of snake venom proteins.