Investigation and biochemical study of novel neurotoxins and thrombo-toxin from the venoms of Elapidae and Viperidae snakes.

蛇毒含有多种有趣的蛋白质，包括促凝剂、抗凝剂、纤溶成分、血小板激动剂、血小板拮抗剂和出血成分。蛇毒的功能成分之一是c型凝集素样蛋白（CLP）家族的成员，包括因子IX/X结合蛋白（IX-X-bp）、carinactivase-1（钙依赖性凝血酶原激活剂）、RVV-X（罗素毒蛇毒液的因子X激活剂）、alboaggreggin -b（血小板激动剂）和flavocetin-A（血小板拮抗剂）。这些clp具有多种活性，并且已经对各种clp进行了测序。clp已被证明是阐明凝血和血小板形成的复杂机制以及人凝血因子和血小板糖蛋白的结构-功能关系的有用工具。从东部水腹蛇（Agkislrodon piscivorus piscivorus）、眼镜王蛇（Ophiophagus hannah）和西部菱形响尾蛇（Crotalus atrox）的毒液中分离、鉴定并克隆了三个新的CRISP家族蛋白（piscivorin、ophanin和catrin）基因。结果表明，CRISP家族蛋白在不同大陆毒蛇科和蛇科的广泛分布，表明CRISP家族蛋白构成了一个新的蛇毒蛋白群。

Snake venom contains a diverse range of interesting proteins, including procoagulants, anticoagulants, fibrinolytic components, platelet agonists, platelet antagonists, and hemorrhagic components. One of the functional components of snake venom is a member of the C-type lectin-like protein (CLP) family that includes factor IX/X-binding protein (IX-X-bp), carinactivase-1 (calcium-dependent prothrombin activator), RVV-X (factor X activator of Russell's viper venom), alboaggregin-B (platelet agonist), and flavocetin-A (platelet antagonist). These CLPs perform a variety of activities, and various CLPs have been sequenced. CLPs have proved to be useful tools in elucidating the complex mechanisms involved in clotting and platelet formation and the structure-function relationship of human clotting factors and glycoproteins of platelets.We isolated, characterized, and cloned genes for three novel CRISP family proteins (piscivorin, ophanin, and catrin) from the venom of eastern cottonmouth (Agkislrodon piscivorus piscivorus), king cobra (Ophiophagus hannah), and western diamondback rattlesnake (Crotalus atrox). Our results show the wide distribution of snake venom CRISP family proteins among Viperidae and Elapidae from different continents, indicating that CRISP family proteins compose a new group of snake venom proteins.