Studies of cell-cell communication during vascular formation

血管形成过程中细胞间通讯的研究

• 批准号: 13440236
• 负责人: FUKUDA Hiroo
• 金额: $ 9.79万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-13440236/
• 关键词: Zinnia elegans; Xylogen; Tracheary element differentiation; Phytosulfokine; Arabidopsis; Cell-cell interaction; Phage display; Mutation; シロイヌナズナ; ブラシノステロイド; 篩部

1. Analysis with Arabidopsis vascular mutants. Van1-van7: We performed cloning of causal genes of Arabidqpsis vascular mutants, vanl-van6 showing fragmented vascular patterns that we have screened. VAN3 encoded a protein regulating the activation of small G-protein. VAN4 encoded a unknown protein, but which may function in membrane traffic. This gene was expressed preferentially hi vascular tissues.2. Analysis of vascular cell differentiation with an in vitro Zinnia system in which mesophyll cells differentiate into xylem cells.(1)We isolated ZePSKl, a homologue of phytosulfokine (PSK) from Zinnia. ZePSKl mRNA accumulated in early and late stages of xylem cell differentiation. Pulse treatment with an inhibitor of PSK biosynthesis and PSK revealed that PSK is necessary for progress of both early and late processes.(2) To identify factors controlling cell-cell communication during vascular cell development, we tried to isolate antibodies recognizing such factors. For such purpose, we used phage display subtraction method that we have developed. Finally we succeeded hi the isolation of about 8000 antibody clones. Tissue staining with randomly selected 90 antibodies revealed that these antibodies recognize apoplastic factors in vascular tissues differently. Thus we could get tools to find out factors involved of cell-cell communication.(3) We succeeded in the isolation encoding "xylogen" which we have identified as a tracheary element-inducing factor.2. Isolation and characterization of a ommature phloem-specific HD-Zip homeobox gene. We isolated a Zinnia HD- Zip class I gene whose mRNA accumulates specifically in immature phloem. Using this gene as a phloem marker, phloem regeneration was analyzed in wounded stems.

1.拟南芥维管突变体分析。VAN1-VAN7：我们克隆了拟南芥维管突变体的致病基因，VAN1-VAN6显示了我们筛选到的零碎的维管模式。VAN3编码一种调节小G蛋白活化的蛋白。VAN4编码一种未知的蛋白质，但可能在膜运输中发挥作用。结论：1.该基因在血管组织中优先表达。百日草叶肉细胞分化为木质部细胞的体外维管细胞分化分析。(1)从百日草中分离到植物硫因子(PSK)的同系物ZePSK1。ZePSK1基因在木质部细胞分化的早期和晚期积累。用PSK生物合成抑制剂和PSK进行脉冲处理表明，PSK对于早期和晚期过程的进展都是必要的。(2)为了确定在血管细胞发育过程中控制细胞间通讯的因素，我们试图分离识别这些因素的抗体。为此，我们使用了我们开发的噬菌体展示差减方法。最终，我们成功地分离了约8000个抗体克隆。用随机选择的90个抗体进行组织染色显示，这些抗体识别血管组织中的质外体因子的方式不同。我们成功地分离到了编码“xylogen”的基因，该基因被鉴定为一种管状分子诱导因子。同源异型盒基因HD-Zip同源框基因的分离与鉴定。我们分离到一个百日草HD-Zip I类基因，它的mRNA在未成熟的韧皮部中特异积累。以该基因为韧皮部标记，对伤茎韧皮部再生进行了分析。

项目成果

Kuriyama, H., and Fukuda, H.: "Developmental Programmed Cell Death in Plants"Curr Opin Plant Biol. 5. 568-573 (2002)

Kuriyama, H. 和 Fukuda, H.：“植物中的发育程序性细胞死亡”Curr Opin Plant Biol。

Endo, M., Matsubara, H., Kokubun, T., Masuko, H., Takahata, Y., Tsuchiya, T., Fukuda, H., Demura, T., Watanabe, M.: "The advantages of cDNA microarray as an effective tool for identification of reproductive organ-specific genes in a model legume, Lotus ja

Endo, M.、Matsubara, H.、Kokubun, T.、Masuko, H.、Takahata, Y.、Tsuchiya, T.、Fukuda, H.、Demura, T.、Watanabe, M.：“cDNA 的优点

Kuriyama, H., and Fukuda, H.: "Programmed cell death in developing tracheary elements"Recent Research Developments in Plant Physiology. 2. 199-212 (2001)

Kuriyama, H. 和 Fukuda, H.：“气管元件发育过程中的程序性细胞死亡”植物生理学的最新研究进展。

Ito, J., Fukuda, H.: "ZEN1 is a key enzyme in degradation of nuclear DNA during programmed cell death of tracery elements"Plant Cell. 14. 3201-3211 (2002)

Ito, J., Fukuda, H.：“ZEN1 是示踪元素程序性细胞死亡过程中核 DNA 降解的关键酶”Plant Cell。

Motose, H: "An arabinogalactan protein(s) is a key component of a fraction that mediates local intercellular communication involved in tracheary element differentiation of zinnia mesophyll cells"Plant Cell Physiol. 42. 129-137 (2001)

Motose, H：“阿拉伯半乳聚糖蛋白是介导参与百日草叶肉细胞气管元件分化的局部细胞间通讯的部分的关键成分”植物细胞生理学。