A basic study on the development of metallic biomaterials

金属生物材料发展的基础研究

• 批准号: 13450050
• 负责人: KOMOTORI Jun
• 金额: $ 6.34万
• 依托单位: KEIO University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-13450050/
• 关键词: Metallic biomaterials; Titanium alloy; Corrosion; Macrophages; SUS316; 生体材料; ステンレス鋼; マクロファージ; 生体適合性; ELID法

Metallic biomaterials are required to have high corrosion resisitan-ce, strength, and biocompatibility.The corrosion relates not only to the biocompatibility of the metallic biomaterials but also to the frac-ture.Therefor we must understand corrosion actions in vivo. But it difficult to elucidate the corrosion phenomenon in vivo. The purpose of this study is to investigate the effects of biological factor on corrosion properties in vitro of metallic implants.This study indecated that the biological factor on corrosion was macrophages in vivo. The corrosion damage of 316 stainless steel disk were performed on seven conditions of immersion test ; that are RPMI1640+FBS, RPMI-1640+FBS+U937 cells, RPMI1640+FBS+Ma-crophages, each medium with high-density polyethylene particles, and Ringer solution. We measured corrosion rate (Rp-1) of SUS316 in each condition by AC. Impedance method and compared their rate. We observed the corrosion process. The results are summarized as follows :1) The surface of SUS316 in medium culturing macrophages at immesion test was incidenced any number of minute protuberances of unevenness. And the average roughness (Ra) and Rp-1 in medium with culturing macrophages increased more than medium without cells, with culturing U937 cells and ringer solution. Therefor these indicated that the existence of macrophages must influence corrosion behavior of bioimplants.2) In case of performing immersion test in medium with macrophages phagocytosed polyethylene particles, the Ra and Rp-1 increased much higher than them in medium with macrophages without phagocytosing. This suggests that the activating macrophages phagocytosed polyethylene particles were sped up corrosion of metallic materials.3) This study suggested the corrosion model in vivo, and it shows that the model can explain a series of experimental results.

金属生物材料要求具有高的耐腐蚀性、强度和生物相容性，腐蚀不仅与材料的生物相容性有关，而且与材料的断裂有关，因此必须了解材料在体内的腐蚀行为。但很难解释体内的腐蚀现象。本研究旨在探讨生物因素对金属植入物体外腐蚀性能的影响，研究结果表明，体内影响金属植入物腐蚀的生物因素是巨噬细胞。在RPMI 1640 +FBS、RPMI-1640 +FBS+U937电池、RPMI 1640+FBS+镁合金、每种介质中加入高密度聚乙烯颗粒和Ringer溶液7种条件下，对316不锈钢圆盘进行了腐蚀损伤试验。我们用交流电测量了SUS 316在各种条件下的腐蚀速率（Rp-1）。阻抗法进行比较。我们观察了腐蚀过程。结果如下：1）在培养巨噬细胞的培养基中，SUS 316细胞表面在浸泡试验中出现了许多不均匀的微小突起。培养U937细胞和林格液的培养基中，培养巨噬细胞的平均粗糙度（Ra）和Rp-1比不含细胞的培养基增加更多。2）在有巨噬细胞吞噬聚乙烯颗粒的介质中进行浸泡试验时，Ra和Rp-1的增加值明显高于无巨噬细胞吞噬的介质。这表明活化的巨噬细胞吞噬聚乙烯颗粒加速了金属材料的腐蚀。3）本研究提出了体内腐蚀模型，表明该模型可以解释一系列实验结果。

项目成果

Yutaka Kameyama: "Effect of FPB(Fine Particles Bombardment) Treatment on the Friction Coefficient of Ti-6A1-4V Alloy"10^<th> World Conference on Titanium, Hamburg, July(2003).

Yutaka Kameyama：“FPB（细颗粒轰击）处理对 Ti-6A1-4V 合金摩擦系数的影响”第 10 届世界钛会议，汉堡，2003 年 7 月。

水谷正義: "金属系生体材料(Ti-6Al-4V合金)の腐食特性に及ぼすELID研削の影響"2002年度精密工学会春季大会学術講演会,東京,3月.

Masayoshi Mizutani：“ELID 磨削对金属生物材料（Ti-6Al-4V 合金）腐蚀特性的影响”2002 年精密工程学会春季会议，东京，3 月。

片平和俊: "ELID研削による表面機能改善(ステンレス鋼の腐食特性の及ぼすELID研削の効果)"精密工学会秋季大会学術講演論文集. 339 (2001)

Shun Katahei：“通过 ELID 磨削改善表面功能（ELID 磨削对不锈钢腐蚀特性的影响）”日本精密工程学会秋季会议论文集 339（2001 年）。

前田澄満: "ポリエチレン粒子を添加したマクロファージ培養液中におけるステンレス鋼の腐食"第48回材料と環境討論会講演集. 489-492 (2001)

Sumimitsu Maeda：“添加聚乙烯颗粒的巨噬细胞培养基中的不锈钢腐蚀”第 48 届材料与环境研讨会论文集 489-492（2001 年）。

Yusuke Goto: "Effect of Plastic Deformation and Static Stress on Corrosion of Ti-6A1-4V Alloy"10^<th> World Conference on Titanium, Hamburg, July(2003).

Yusuke Goto：“塑性变形和静态应力对 Ti-6A1-4V 合金腐蚀的影响”第 10 届世界钛会议，汉堡，2003 年 7 月。