Functional domain identification of transcription factors regulating expression of fungal polysaccharide-degrading enxyme genes.

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1. AmyR A. nidulans AmyR bound to DNA fragments containing CGGN_8(C/A)GG. Binding of two AmyR molecules to the consensus sequence was required for transcriptional activation of the agdA and taaG2 genes. AmyR shares 5 homologous regions with yeast Mal activators (Zn and MH1-4 from N-terminus). MH4 was required for signal-dependent regulation of AmyR activity and MH2 was required for a transcriptional activation.2. X1nR A.oryzae X1nR bound to not only GGCTAA but also GGCTGA containing sequence. AoXlnR controlled expression of both xylanolytic and cellulolytic enzyme genes. In addition to xylan, cellulose could induce X1nR-dependent transcription.3. Hap complex A.oryzae Hap complex at least contains HapB, C, and E as subunits. Assembly of the core regions from each subunit was sufficient for DNA binding. However, for transactivation of Hap-regulated genes, the N-terminal region of HapC and the C-terminal region of HapB were also required. HapC was required for HapE stability. In addition a new factor HapX was identified as a protein which interacted with the Hap complex.