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Functional domain identification of transcription factors regulating expression of fungal polysaccharide-degrading enxyme genes.

调节真菌多糖降解酶基因表达的转录因子的功能域鉴定。

基本信息

批准号：
13460038
负责人：
KOBAYASHI Tetuo
金额：
$ 5.95万
依托单位：
Nagoya University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2001
资助国家：
日本
起止时间：
2001 至 2002
项目状态：
已结题

项目摘要

1. AmyR A. nidulans AmyR bound to DNA fragments containing CGGN_8(C/A)GG. Binding of two AmyR molecules to the consensus sequence was required for transcriptional activation of the agdA and taaG2 genes. AmyR shares 5 homologous regions with yeast Mal activators (Zn and MH1-4 from N-terminus). MH4 was required for signal-dependent regulation of AmyR activity and MH2 was required for a transcriptional activation.2. X1nR A.oryzae X1nR bound to not only GGCTAA but also GGCTGA containing sequence. AoXlnR controlled expression of both xylanolytic and cellulolytic enzyme genes. In addition to xylan, cellulose could induce X1nR-dependent transcription.3. Hap complex A.oryzae Hap complex at least contains HapB, C, and E as subunits. Assembly of the core regions from each subunit was sufficient for DNA binding. However, for transactivation of Hap-regulated genes, the N-terminal region of HapC and the C-terminal region of HapB were also required. HapC was required for HapE stability. In addition a new factor HapX was identified as a protein which interacted with the Hap complex.

1. AmyR 构巢菌 AmyR 与含有 CGGN_8(C/A)GG 的 DNA 片段结合。 agdA 和 taaG2 基因的转录激活需要两个 AmyR 分子与共有序列结合。 AmyR 与酵母 Mal 激活剂（N 末端的 Zn 和 MH1-4）共享 5 个同源区域。 AmyR活性的信号依赖性调节需要MH4，转录激活需要MH2。2． X1nR 米曲霉 X1nR 不仅与 GGCTAA 结合，还与含有 GGCTGA 的序列结合。 AoXlnR 控制木聚糖分解酶和纤维素分解酶基因的表达。除木聚糖外，纤维素也能诱导X1nR依赖性转录。3． Hap复合体A.oryzae Hap复合体至少包含HapB、C和E作为亚基。每个亚基的核心区域组装足以进行 DNA 结合。然而，为了反式激活 Hap 调节基因，还需要 HapC 的 N 端区域和 HapB 的 C 端区域。 HapC 是 HapE 稳定性所必需的。此外，一个新的因子 HapX 被鉴定为与 Hap 复合物相互作用的蛋白质。