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Studies on the Water Purification in the Coastal Areas of Inner Bay Using Nitrifying and Denitrifying Bacteria in the Tidal Bottom-Mud

内湾沿岸地区潮底泥硝化反硝化细菌净化水质的研究

基本信息

批准号：
13460107
负责人：
SEGUCHI Masahiro
金额：
$ 7.42万
依托单位：
Saga University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2001
资助国家：
日本
起止时间：
2001 至 2003
项目状态：
已结题

项目摘要

The purposes of the present research are to clarify the relationships between the biological activity of nitrifying and denitrifying bacteria and various environmental factors in the tidal bottom-mud, and to use the nitrification and denitrification processes maintained by the metabolism of these bacteria in order to purify water of the coastal areas in the inner bay. The conclusions obtained by the present research during three years from 2001 to 2002 are summari zed as follows :(1)A kind of nitrifying bacteria and two kinds of denitrifying bacteria with high biological activity were selected and isolated from the tidal bottom -mud i n the Ariake Sea. Moreover, the properties of these bacteria were clarified.(2)The relationships between the nitrification and denitrification rates in the tidal bottom -mud and control factors such as substrate concentr ation, temperature, pH, reduction potential were clarified. A Mechaelis -Menten equation was valid for the relation between nitrification and denitrification rates, substrate concentration and temperature. This equation indicated the optimum temperature for nitrification and denitrification rates and these Timit values.(3)A method for removal of inorganic nitrogen compounds i n the waste water using the tidal bottom-mud. where many nitrifying and denitrifing bacteria with high biological activity lived was proposed. Moreover, a trial device for its removal based on this method was made.(4)The relationships between N-removal rates of this trial device and NH_4^+ concentration, temperature, flow, mud-content, pH and number of nitrifying and denitrifying bacteria were commanded experimentally.(5)The utility of the removal device was verified and the extent power of water purification of the tidal bottom -mud in the Ariake Sea was confirmed. On the other hand, the issues for the practical use of the removal device were also found.

本研究旨在阐明潮汐底泥中硝化细菌和反硝化细菌的生物活性与各种环境因素之间的关系，并利用这些细菌的新陈代谢所维持的硝化和反硝化过程来净化内湾沿岸地区的水质。本研究在2001~2002年的三年时间里，取得了以下主要研究成果：(1)从阿里亚克海潮间带底泥中筛选出一株具有较高生物活性的硝化细菌和两株反硝化细菌。阐明了潮汐底泥中硝化和反硝化速率与底物浓度、温度、pH、还原电位等控制因素之间的关系。硝化作用与反硝化速率、底物浓度和温度之间的关系符合Mechaelis-Menten方程。该方程给出了硝化和反硝化速率的最佳温度和这些Timit值。(3)潮汐底泥去除废水中无机氮化合物的方法。提出了具有较高生物活性的硝化细菌和反硝化细菌的栖息地。实验掌握了该装置的脱氮效率与NH_4~+浓度、温度、流量、含泥量、pH值、硝化细菌和反硝化细菌数量的关系。(5)验证了该装置的实用性，证实了该装置对阿里亚克海潮间带底泥的净水能力。另一方面，也发现了拆卸装置在实际使用中存在的问题。