Development of Recombined Plants Enzyme Genes for Product Useful Compound

研究了外源酶基因对次生代谢物质合成的影响。Duboisia和天仙子H6 H-基因，代谢莨菪碱为东莨菪碱。对12个含有H6 H基因的杜氏木细胞系和8个天仙子细胞系进行了诱导。讨论了它们合成东莨菪碱的能力。结果表明：1）在东莨菪碱的合成中，盾叶薯蓣属的部分细胞株表现出较高的东莨菪碱合成量，天仙子属的部分细胞株表现出较高的东莨菪碱合成量;在18个杜波依属细胞系和13个甜菜属细胞系中存在代谢阿魏酸为香草醛的HCHL基因（2）。然而，他们的基因在前者中没有被揭示。在后者中，茉莉酸甲酯诱导其基因产生mRNA，而4-羟基苯甲酸和香草酸诱导其基因产生mRNA，但不产生香草醛。HMGR是该途径中的关键酶，受HMGR激酶的调控。将HMGR激酶基因（NPK 5）、NPK 5-K48 R基因（4）和糖苷酶基因（5）导入该植物。但没有得到含有这些基因（3，4）的细胞系。基因修饰的糖苷酶基因（5）抑制甾体皂苷的生成。

An Influence of external genes which synthesis enzyme, on Second metabolic substance productions were studied.1)Duboisia leichhardtii and Hyoscyamus albus : We introduced to. Duboisia and Hyoscyamus H6H-gene which metabolize hyoscyamine to scopolamine. 12 cell lines of Duboisia and 8 cell lines of Hyoscyamus which had H6H gene were induced. Their ability, of product scopolamine were discussed. Some cell lines in Duboisia indicated higher scopolamine product and Some cell lines in Hyoscyamu indicated higher hyoscyamine product : Two new unknown alkaloids were found In the latter some cell lines.2)D. leichhardtii and Beta vulgaris HCHL gene(2) which metabolize ferulic acid to vanillin existed in 18 cell lines of Duboisia and 13 cell lines of Beta. However their genes were not revealed in the former. In the latter, m-RNA induced from their genes existed by methyl jasmonate treatment, and 4-hydroxybenzoic acid and vanillic acid product but did not vanillin.3)Solanum aculeatissimum : this plant product glygoside via pathway acetic acid-rnebaronic acid. HMGR is key enzyme in the pathway was controled by HMGR-kinase. HMGR-kinase (NPK5) gene (3)NPK5-K48R gene (4)which was modified NPK5 and glycoside enzyme gene(5) were introduced to this plant. But cell line contained these genes(3,4) did not gotten. The, gene modified glycoside enzyme gene(5) inhibited steroidal saponin product.