Production of allelochemics by plant tissue culture and development of these subsutances

一枝黄花根茎和根中含有大量的聚乙炔类化感物质，如顺式-去氢母菊酯（cis-DME）、反式-去氢母菊酯（trans-DME）、顺式-母菊酯（cis-ME）、反式-母菊酯（trans-ME）、顺式-毛叶树酯（cis-LE）、10-[（2）-2-甲基-2-丁烯酰氧基]-（2Z，8Z）-2，8-癸二烯-4，6-二炔酸甲酯。它们的物质抑制另一种植物的生长。本研究利用植物组织培养的方法生产其活性物质，并开发其除草剂活性物质.物质的提取和结晶：根茎和根的干燥粉末用甲醇提取。甲醇提取物经柱层析分离。根据分析结果，得到了两种红色晶体，通过1H-NMR光谱确定为trans-DME和cis-DME。10-[（2）-2-甲基-2-丁烯酰氧基]-（2Z，8Z）-2，8-癸二烯-4，6-二炔酸甲酯经~1H-NMR谱确认，但不能以晶体形式分离 关于我们 stal.2.毛状根的诱导和增加：用发根农杆菌菌株ATCC 15834接种无菌小植株，诱导该植物的毛状根。许多不定根出现，但他们没有增加。将这些不定根培养在MS或Gamborg基本培养基上，添加赤霉素或萘乙酸（NAA），但它们不生长。因此，它们是否是毛状根，无法检验。愈伤组织的诱导和增殖：将幼苗上胚轴培养在MS +1ppm 2，4D +2ppm苄基腺嘌呤的基本培养基上，可诱导出愈伤组织。诱导愈伤组织在MS附加1ppm NAA和1ppm激动素的培养基上生长旺盛。培养4周的愈伤组织中，cis-DME的含量为微量.野生植物根茎和根中物质的产生：推测野生植物根茎和根中物质的产生因生长期而异，然后从5月至12月每月调查一次其中的顺式DME量。检测结果表明，10月至11月是一年中最高的月份，是5月最低的月份的15倍。少

Solidago altissima contains high levels of the poliacetylenes, cis-dehydromatricaria ester (cis-DME), trans-dehydromatricaria ester (trans-DME), cis-matricaria ester (cis-ME), trans-matricaria ester (trans-ME), cis-Lachnophyllum ester (cis-LE), methyl 10-[(2)-2-methyl-2-butenoyloxy]-(2Z, 8Z)-2, 8-decadiene-4, 6-diynoate, as allelopathic substances in the rhizomes and roots. Their substances inhibit growth of another plants. We studied to product their substances by plant tissue culture and to develop their substances for herbicide.1. Extract and crystallize of substances : Dried powder of the rhizomes and roots were extracted with methanol. Methanol extract was separated by column chromatography. On the result of analysis, two red color crystals, which were confirmed trans-DME and cis-DME by 1H-NMR spectrometry, were obtained. And also, methyl10-[(2)-2-methyl-2-butenoyloxy]-(2Z, 8Z)-2, 8-decadiene-4, 6-diynoate was recognized by ^1H-NMR spectrometry but it could not be separated as cry … More stal.2. Induce and increase of hairy roots : Hairy roots of this plant were initiated with sterilized plantlets inoculated with Agrobacterium rhizogenes strain ATCC15834. Many adventitious roots emerged, but they did not increased. These adventitious roots were cultivated on Murashige and Skoog (MS) or Gamborg's basal medium, added gibberellin or naphthaleneacetic acid (NAA) but they did not grow. Therefore, whether they were hairy roots or not could not be examined.3. Induce and increase of callus : When the epicotyl of seedling was cultivated on MS basal medium containing 1 ppm 2, 4D and 2ppm benzyladenine, callus were induced. Induced callus very much grew with MS basal medium added 1ppm NAA and 1ppm kinetin. Amount of cis-DME in callus cultivated for 4 weeks was trace level.4. Production of substances in the rhizomes and roots of wild plants : It was guessed that production of substances in the rhizomes and roots of wild plants differ from growth period and then amount of cis-DME in them were investigated once a month from May to December. The results of examination, its amount was highest on October to November in a year whose amounts showed 15th times than lowest on May. Less