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BREEDING OF HIGH DIGESTIBLE RICE PLANT FOR FEED USING SOMACLONAL VARIATION.

利用体细胞克隆变异育种高消化率饲料稻植物。

基本信息

批准号：
13460119
负责人：
KAWAMURA Osamu
金额：
$ 9.54万
依托单位：
University of Miyazaki
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2001
资助国家：
日本
起止时间：
2001 至 2003
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-13460119/
关键词：
BREEDING CULTIVATION OF CALLI SUSPENSION CULTURE HIGH DIGESTIBILITY SOMACLONAL VARIATION RUMINANT FEED RICE PLANT

项目摘要

In order to produce the high quality rice plant for ruminant feed by the tissue culture technique, we carried out the experiments as follows ;The calli were induced from the scutellum of matured rice seed (cv. Nihonbare). At 28 days after initial cultivation, the embryogenic structures covered with the compact callus was formed. The successive cultivation regenerated the 89 plants from the re-differentiation medium through the formation of somatic embryogenesis.Fifty-three regenerated plans were randomly selected, transplanted into pots (1/5000a) and transferred to the outdoors. Thirty-seven control plants, which were obtained from the individual seed of Nihonbare, were grown under the same conditions. The regenerated plants have a tendency to be late for the ear emergence. Additionally, they have more number of ear, less seeds weight and lignin content and higher leaf/stem ratio, compared with the control. It was not observed the significant differences in the plant length, the dry ma … More tter yield, the dry matter digestibility and the digestible dry matter yield between the regenerants and the control. In the most of measurements, the range of values is wider in the regenerant than in the control and the distribution pattern is different between two groups.Successively, the promising 3 lines were selected from the regenerated plants and were grown under the same condition above. The term of ear emergence of in the regenerant group is longer than the control groups. One of 3 lines produced more the digestible dry matter than the control groups. The digestible dry matter yields were more variable in the regenerant group. The weights of seed in the regenerants were equivalent or less compared with control. In the most of measurements, the range of values is wider in the regenerant than in the control and the distribution pattern is different between two groups. It was recognized that some plants in the regenerant group have the much seed and digestible dry matter in leaf and stem, and have the remarkable digestible dry matter though having less seed. We obtained the higher digestible rice plants in the regenerant plants than in the control "Nihonbare", but are required the successive experiment because the trait is not fixed yet. Less

为了利用组织培养技术生产出优质的反刍动物饲料用水稻植株，我们进行了以下试验：以成熟水稻种子(cv。Nihonbare)。培养28 d后，形成覆盖致密愈伤组织的胚性结构。经连续培养，89株植株在再分化培养基上通过体细胞胚发生再生。随机选择53个再生平面，移植到花盆中（1/5000a），并转移到室外。在相同的条件下，从日本山竹单株种子中获得37株对照植株。再生植株有穗出晚的趋势。此外，与对照相比，它们的穗数更多，种子重量和木质素含量更少，叶/茎比更高。再生剂在植株长度、干物质产量、干物质消化率和可消化干物质产量方面与对照无显著差异。在大多数测量中，再生剂的数值范围比对照组更宽，两组之间的分布模式不同。依次从再生植株中选择有潜力的3个品系，在相同条件下进行培养。再生组的出耳期明显长于对照组。3个品系中有一个品系的可消化干物质产生量高于对照组。再生剂组的可消化干物质产量变化较大。再生植株的种子质量与对照相当或低于对照。在大多数测量中，再生剂的数值范围比对照组更宽，两组之间的分布模式不同。结果表明，再生组中部分植物叶片和茎中种子和可消化干物质含量较高，种子含量较低，但可消化干物质含量显著。我们在再生植株中获得了比对照“二本裸”更高的消化率，但由于性状尚未固定，需要进行连续试验。少