权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Effects of appetite regulating nuropeptides on somatotroph axis of cattle

食欲调节神经肽对牛生长激素轴的影响

基本信息

批准号：
13460122
负责人：
YAMAGUCHI Takahiro
金额：
$ 9.09万
依托单位：
Tohoku University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2001
资助国家：
日本
起止时间：
2001 至 2003
项目状态：
已结题

项目摘要

Expression and affects of appetite regulating nuropeptides (orexin : OX, Galanin : Gal, leptin : Lep, nuropeptide Y : nero Y) on somatotrophs were detailed in bovine adenohypophysis. Prepro-OX mRNA and OX1-receptor (OX1R) mRNA were expressed and their partial DNA and amino acid sequences were determined in bovine adenohypophysis. OX-A was localized in PRL cells and OX1R was also detected in the identical cell type. However, OX-A was not effective on the release of GH and PRL in the primary cultures of the adenohypophysial cells. Gal mRNA and immunoreactive Gal were localized in ACTH cells. The Gal expression was mainly mediated by testosterone. Gal producing cells also expressed both of androgen receptor (AR) and estrogen receptor (ER). It was demonstrated that the Gal expression in ACTH cells was regulated by ER-binding signals of estrogen derived from testosterone by P450 aromatase in another type cells of the adenohypophysis, so called, intracrinology fashion Lap mRNA were expressed in the adenohypophysis and immunoreactive Lep was mainly localized in GH cells although was immunoreactive in a small number of PRL, TSH and GTH cells. Since cytokines are known to exert important roles in nuroendocrine system, the expression of interleukin 18 (IL-18) and its receptor (IL-18R) in the adenohypophysial cells was examined in detail. IL-18 mRNA and immunoreactive IL-18 were localized in GH cells. The partial DNA sequence of bovine IL-18R α was determined and immunoreactive IL-18Rα was also localized in GH cells. These findings indicate that IL-18 may plays GH secretion or the differentiation of somatotrophs in autocrine fashion. Furthermore, bovine adenohypophysial cell line that presents IL-18 and IL-18Rα,and can passages for long time was established. New findings in the present study are ordering to submit international Journals as four publications.

本文详细介绍了食欲调节神经肽(食欲素：OX、甘丙肽：GAL、瘦素：LEP、神经肽Y：Nero Y)在牛腺垂体中的表达及其对生长激素的影响。在牛腺垂体中表达了前OX基因和OX1受体(OX1R)基因，并测定了它们的部分DNA和氨基酸序列。OX-A定位于PRL细胞，OX1R也定位于同一细胞类型。但OX-A对原代培养的腺垂体细胞释放GH和PRL无明显影响。ACTH细胞内有Gal mRNA和Gal免疫反应阳性产物。Gal的表达主要受睾酮的调节。GAL产生细胞同时表达雄激素受体(AR)和雌激素受体(ER)。结果表明，ACTH细胞中Gal的表达受另一种类型的腺垂体细胞中P450芳香化酶所产生的雌激素的ER结合信号的调节，即腺垂体中有Lap mRNA的表达，Lep免疫反应主要定位于GH细胞，少数PRL、TSH和GTH细胞也有Lep表达。鉴于细胞因子在神经内分泌系统中的重要作用，本研究对腺垂体细胞中白介素18(IL-18)及其受体(IL-18R)的表达进行了详细研究。IL-18mRNA和免疫反应阳性的IL-18定位于GH细胞。测定了牛IL-18Rα的部分序列，并将免疫反应阳性的IL-18Rα定位于生长激素细胞。提示IL-18可能以自分泌方式参与生长激素的分泌或促生长激素细胞的分化。此外，建立了表达IL-18和IL-18Rα，并能长时间传代的牛腺垂体细胞系。本研究的新发现要求将《国际期刊》作为四种出版物提交。