Accumulation of primary data for construction of database in wild-rice genetic resources collected in tropical Asia.

建立野生稻基因库资源数据库是水稻育种利用的必要条件。因为这些野生水稻是遗传资源。本研究项目的目的是建立在亚洲热带和亚热带地区收集的野生水稻品种数据库。本研究项目的主要目的是积累有关形态、生理和分子标记的原始数据，以及在居住地的实地数据，具体如下：1. 野生稻采集点野外资料（经纬度、海拔、野生稻受扰情况、水土状况）。等)。2. 种子的形态资料和幼苗的生理资料（发芽、休眠、耐温等）。等)。3. 野生稻3^<rd>内含子区线粒体DNA和g -重复序列多态性分析4. 野生水稻品系间叶绿素DNA多态性分析。5. 体细胞染色体上45SeRNA基因数目的多样性。6. 野生水稻AA基因组种间叶绿素DNA微卫星多态性分析。6. 野生水稻种子可视化数据库（稻壳颜色、芒的长度和颜色、顶端毛的密度和长度、颖片的长度）。

The construction of database concerning the wild rice resources stored in gene banks is necessary for the utilization of rice breeding. because these wild rices are genetic recourses. The purpose of this research project supported by Grant-in Aid is to construct the database of wild rice strains collected in tropical and subtropical Asia. The primary goal of this research project was to accumulation of primary data concerning morphological, physiological and molecular markers in addition to field data in habitation site, as follow. 1. Field data of collection sites in wild rices (latitude, longitude, elevation, disturbance of wild rice, water and soil condition. et al.). 2. Morphological data of seed and physiological data of seedling (germination, dormancy, temperature tolerance. et al.). 3. Polymorphism of mitochondrial DNA and G-repeat in 3^<rd> intron region for the distinction among wild rice species. 4. Polymorphism of chlorophyll DNA for the distinction among wild rice strains. 5. Diversity of 45SeRNA gene number on chromosomes in somatic cells. 6. Polymorphism of microsatellite in chlorophyll DNA for the distinction among AA genome species in wild rice. 6. Visual database of wild rice seeds (hull color, length and color of awn, density and length of apical hair, length of glumes).