MicroRNAs associated with smoking-induced pulmonary emphysema in senescence marker protein-30 knockout mice

MicroRNA与衰老标记蛋白30敲除小鼠吸烟引起的肺气肿相关

• 批准号: 23790922
• 负责人: SATO Tadashi
• 金额: $ 2.75万
• 依托单位: Juntendo University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23790922/
• 关键词: 慢性閉塞性肺疾患(COPD); microRNA; miR-146a; inflammation; smoking; SMP30; マイクロRNA; 喫煙; SMP30KOマウス; 炎症; SMP30ノックアウトマウス; マイクロアレイ解析; SMP30ﾉｯｸｱｳﾄﾏｳｽ

Cigarette smoking is the most important cause of chronic obstructive pulmonary disease (COPD), but the mechanisms of pathogenesis are incompletely defined. We have previously reported that senescence marker protein-30 knockout (SMP30-KO) mice can be useful for investigating cigarette smoke-induced pulmonary emphysema. Moreover, we have already shown that miR-146a plays a pathogenetic role in the abnormal inflammatory response in COPD.Vitamin C-controlled SMP30-KO were given miR-146a inhibitor on day 1 and 8 intranasally during exposure to either diluted cigarette smoke or fresh air for 2 weeks. Total cell and lymphocyte numbers were increased in the BALF of both miR-146a inhibitor and smoke exposed mice. PGE2 production and COX-2 expression were also increased significantly higher by exposure both miR-146a inhibitor and cigarette smoke.In conclusion, miR-146a appears to play a pathogenetic role in the abnormal inflammatory response in a cigarette smoke-exposed murine model of COPD.

吸烟是慢性阻塞性肺疾病(COPD)的重要病因，但其发病机制尚不完全清楚。我们以前曾报道过衰老标记蛋白-30基因敲除(SMP30-KO)小鼠可用于研究香烟烟雾诱导的肺气肿。此外，我们已经证明miR-146a在COPD的异常炎症反应中起致病作用。维生素C控制的SMP30-KO在暴露于稀释香烟烟雾或新鲜空气中的第1天和第8天鼻内给予miR-146a抑制剂2周。MiR-146a抑制剂组和烟雾暴露组小鼠肺泡灌洗液中细胞总数和淋巴细胞数均增加。暴露于香烟烟雾暴露的COPD小鼠模型中，暴露于miR-146a抑制剂和香烟烟雾均显著增加PGE2的产生和COX-2的表达。

项目成果

慢性閉塞性肺疾患(COPD)の発症と病態に関与するmicroRNA-SMP30ノックアウトマウスを用いた解析-

使用 microRNA-SMP30 敲除小鼠对慢性阻塞性肺疾病 (COPD) 的发病和病理学进行分析

• 发表时间: 2013
• 作者: Minoru Inomata;Koichiro Kamio;Kuniko Matsuda;Nariaki Kokuho;Yukiko Miura;Kazue Fujita;Yoshinobu Saito;Akihiko Gemma;Arata Azuma;佐藤匡
• 通讯作者: 佐藤匡

Annual Review 呼吸器

呼吸系统年度审查

• 发表时间: 2013
• 作者: 野田雅史;岡田克典;近藤丘;野田雅史 岡田克典 近藤丘
• 通讯作者: 野田雅史 岡田克典 近藤丘

The p38 mitogen-activated protein kinases modulate endothelial cell survival and tissue repair

• DOI: 10.1007/s00011-011-0405-7
• 发表时间: 2012-03-01
• 期刊: INFLAMMATION RESEARCH
• 影响因子: 6.7
• 作者: Kanaji, Nobuhiro;Nelson, Amy;Liu, Xiangde
• 通讯作者: Liu, Xiangde

新しい診断と治療のABC(1)慢性閉塞性肺疾患

新诊治基础知识（一）慢性阻塞性肺疾病

• 作者: 佐藤匡;他
• 通讯作者: 他

MicroRNAs associated with smoking-induced pulmonary emphysema in senescence marker protein-30 knockout mice

MicroRNA与衰老标记蛋白30敲除小鼠吸烟引起的肺气肿相关

• 发表时间: 2014
• 作者: 猪俣稔;神尾孝一郎;松田久仁子;國保成暁;根井貴仁;三浦由記子;藤田和恵;齋藤好信;弦間昭彦;吾妻安良太;佐藤匡
• 通讯作者: 佐藤匡