Molecular design and construction of infection protective antigens for Q fever agent

Q热剂感染保护性抗原的分子设计与构建

• 批准号: 13460142
• 负责人: MASEGI Toshiaki
• 金额: $ 9.86万
• 依托单位: Gifu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-13460142/
• 关键词: Coxiella burnetii; pathogenicity; pathogen factor, SCID mice; 分子疫学; コクシエラ菌の感染防御抗原; コクシエラ菌の分子設計; コクシエラ菌の分子構築; Q熱リケッチアの分子構築

We investigated differences among Coxiella burnetii strains using a new animal experiment model of SCID mice for Q fever. Pathogenicity of a chronic strains in SCID mice was completely different from that of the acute strain. The severity of the heart lesion in SCID mice caused by C. burnetii Priscilla strain, the prototype strain for chronic Q fever, were less severe than expected. The clinical course of SCID mice infected with Priscilla strain was long and mild, and the histopathological observations were mild. The results suggest that pathogen factors as well as host factors are involved in the manifestation of Q fever.Then we investigated PCR-RFLP on icd nad, com1 genes for 72 isolates in Japan. Fourty-nine isolates showed varied PCR-RFLP, which was reported on isoates overseas, to other isolates which showed the same profile as published pattern on icd gene. Sequences of the varied isoates in Japan were different from those of published data. Putative amino acid sequences of the varied isolates in Japan were identical to those of plasmid-less choric strains~. We showed the presence of unique strains in Japan.Our project resulted in the usefulness of SCID mice for an animal model of Q fever, the establishment of Latex agglutination assay for detecting antigen and antibody of Q fever, and molecular properties of C. burnetii isolates in Japan which should be different from those of overseas isolates.

我们使用一种新的Q热的SCID小鼠动物实验模型来研究伯氏柯克斯体不同菌株之间的差异。一株慢性毒株对SCID小鼠的致病性与急性毒株完全不同。慢性Q热的原型菌株伯氏梭菌普里西拉株对SCID小鼠造成的心脏损害没有预期的严重。感染Priscilla株的SCID小鼠临床病程长而轻，组织病理学观察较轻。结果表明，Q热的表现与病原因素和宿主因素有关。然后，我们对72株日本分离株的ICD、NAD、COM1基因进行了PCR-RFLP分析。49株分离物的PCR-RFLP图谱与国外报道的不同，与已发表的ICD基因图谱相同。日本的各种异构体的序列与已发表的数据不同。日本不同分离株的氨基酸序列与无质粒的合体株完全相同。我们的项目使SCID小鼠可用于Q热的动物模型，建立了检测Q热抗原和抗体的乳胶凝集试验，以及日本分离的布氏梭菌的分子特性，这应该与国外的分离株有所不同。

项目成果

Andoh, M.et al.: "SCID mouse model for lethal Q fever"Infect.Immun.. 71. 4717-4723 (2003)

Andoh, M.等人：“致死性 Q 热的 SCID 小鼠模型”Infect.Immun.. 71. 4717-4723 (2003)

Hotta, A.: "Use of monoclonal antibodies to lipopolysaccharide for antigenic analysis of Coxiella burnetii"J.Clin.Microbiol.. 41・4. 1747-1749 (2003)

Hotta，A.：“使用针对脂多糖的单克隆抗体进行伯氏立克次体的抗原分析”J.Clin.Microbiol.. 1747-1749 (2003)。

Ikuta, K.: "Diagnostic evaluation of 2',5'-oligoadenylate synthetase activities and antibodies against Epstein-Barr virus and Coxiella burnetii in patients with chronic fatigue syndrome in Japan"Microbes Infect.. 5. 1096-1102 (2003)

Ikuta, K.：“日本慢性疲劳综合征患者的 2,5-寡腺苷酸合成酶活性和抗 Epstein-Barr 病毒和伯氏柯克斯体抗体的诊断评估”Microbes Infect.. 5. 1096-1102 (2003)

Komiya, T.: "Seroprevalence of Coxiella burnetii infections among cats in different living environments"J.Vet.Med.Sci.. 65. 1047-1048 (2003)

Komiya, T.：“不同生活环境中的猫中伯氏柯克斯体感染的血清流行率”J.Vet.Med.Sci.. 65. 1047-1048 (2003)

Ikuta, K.et al.: "Diagnostic evaluationo f 2'-, 5'-oligoadenylate synthetase activities and antibodies against Epstein-Barr virus and Coxiella burnetii in patients with chronic fatigue syndrome in Japan"Microbes Infect.. 5. 1096-1102 (2003)

Ikuta, K. 等人：“日本慢性疲劳综合征患者的 2-, 5-寡腺苷酸合成酶活性和抗 Epstein-Barr 病毒和伯内特柯克斯体抗体的诊断评估”微生物感染.. 5. 1096-1102