DEVELOPMENT OF BIOCOMPATIBLE GRAFTS WITH AUTOLOGOUS VASCULAR CELLS

自体血管细胞生物相容性移植物的开发

• 批准号: 13470268
• 负责人: YAMAMOTO Fumio
• 金额: $ 8.7万
• 依托单位: Akita University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-13470268/
• 关键词: Tissue Engineering; Biodegradable Vascular Graft; Cell Transplantation; ティシュエンジニアリング; Hybrid Graft; 内皮細胞; 平滑筋細胞; 線維芽細胞; 人工血管; 生体吸収性; ハイブリッド

The artificial vascular graft has potential disadvantages that relate to use of a conduit, including lack of growth potential and thromboembolism for children.A biocompatibly structured vascular graft, resembling the multilayered structure of a natural vessel, may accelerate to regenerate the physiological function and growth potential of similar to those of a natural vessel. In this study, we developed biocompatible vascular grafts incorporated with autologous endothelial cells (ECs), smooth muscle cells (SMCs) and fibroblasts (FCs) on a biodegradable polymer.Biocompatible vascular grafts were constructed on tubular scaffolds (ID=5mm, Length; 6cm) in vitro. Tubular scaffolds were made of biodegradable polymers composed of a polyglycolic acid (PGA), and a copolymer of L-lactide and aprolactone. Canine vascular cells were isolated from their jugular veins, and grown in cell cultures. The grafts consisted of ECs confluent monolayer and homogeneously mixed SMCs and labelled FCs layer. The designed artificial ECM was a mixed gel of fibrin glue.Pre-implanted grafts were completely endothelialized by scanning electron microscopy (SEM). Grafts have been implanted bilaterally in canine carotid arteries without anticoagulant. These grafts were achieved at physiological perfusion pressures.Vascular cells incorporated into biodegradable artificial grafts may contribute to enhanced tissue regeneration process including antithrombogenicity and growth potential.

人造血管移植物具有与导管的使用相关的潜在缺点，包括缺乏生长潜力和儿童血栓栓塞。生物相容性结构的血管移植物，类似于天然血管的多层结构，可以加速再生类似于天然血管的生理功能和生长潜力。在本研究中，我们将自体内皮细胞（EC）、平滑肌细胞（SMC）和成纤维细胞（FC）复合于生物可降解聚合物上，在体外构建了生物相容性血管移植物。管状支架由可生物降解的聚合物制成，该聚合物由聚乙醇酸（PGA）和L-丙交酯和丙内酯的共聚物组成。从犬颈静脉分离血管细胞，并在细胞培养物中生长。移植物由单层融合的内皮细胞、均匀混合的平滑肌细胞和标记的FC层组成。所设计的人工ECM为纤维蛋白胶的混合凝胶，扫描电镜（SEM）显示移植物完全内皮化。移植物已被植入双侧犬颈动脉无抗凝剂。这些移植物是在生理灌注压力下获得的，血管细胞掺入可生物降解的人工移植物可能有助于增强组织再生过程，包括抗血栓形成和生长潜力。

项目成果

Kazuyuki Ishibashi: "Macromolecular Assemblies in Hybrid Vascular Graft Reconstructed with Three Cell Types"Akita Journal of Medicine. Vol.29. 123-134 (2002)

Kazuyuki Ishibashi：“用三种细胞类型重建混合血管移植物中的大分子组件”秋田医学杂志。

Kazuyuki Ishibashi, Fumio Yamamoto: "Macromolecular Assembles in Hybrid Vascular Graft Reconstructed with Three Cell Types"Akita J.Med.. Vol.29 No.2. 123-134 (2002)

Kazuyuki Ishibashi、Fumio Yamamoto：“用三种细胞类型重建混合血管移植物中的大分子组装”Akita J.Med.. Vol.29 No.2。

Kazuyuki Ishibashi: "Macromolecular Assemblies in Hybrid Vascular Graft Reconstructed with Three Cell Types"Akita J Med. Vol.29 No.2. 123-134 (2002)

Kazuyuki Ishibashi：“用三种细胞类型重建混合血管移植物中的大分子组件”Akita J Med。

Yoshifumi Chida: "Development of Biocompartible Vascular Graft Using Autologous Vascular Cells And Biodegradable Scaffold in Systemic Circulation System"ASAIO J. Vol.50(in press). (2004)

Yoshifumi Chida：“在全身循环系统中使用自体血管细胞和生物可降解支架开发生物相容性血管移植物”ASAIO J. Vol.50（印刷中）。

Yoshifumi Chida: "Development of Biocompartible Vascular Graft Using Autologous Vascular Cells And Biodegradable Scaffold in systemic Circulation System"ASAIO Journal. Vol.50(in press). (2004)

Yoshifumi Chida：“在全身循环系统中使用自体血管细胞和生物可降解支架开发生物相容性血管移植物”ASAIO 杂志。