Therapeutic Cell-transplantation for Sever Heart Failure

本研究旨在发展细胞心肌成形术治疗严重心力衰竭的理想程序。胎儿心肌细胞（FCM）移植可预防但不能逆转慢性心肌梗死大鼠的心脏重构。然而，结合bfgf微球的预血管化可以增强FCM移植的益处。FCM移植对左室后期扩张和左室修复术后功能障碍有预防作用。自体细胞，如骨骼肌母细胞（SM）和骨髓干细胞（BMSC），对缺血的耐受性比CM强。在SM的研究中。在心肌梗死大鼠中，大量新鲜分离的新生SM能够在宿主心脏中存活，并能以逆行左室重构完全替代梗死心肌。静脉（IV）和心肌（IM）输送BMSC移植。在心肌梗塞模型和扩张型心肌病（DCM）模型中对小鼠进行超声心动图和组织学评价。移植分为急性期和慢性期。在急性期，骨髓间充质干细胞移植的IV和IM给药在MI和DCM模型中都是有效的，而在慢慢期，IM给药在。DCM模型中静脉给药效果优于MI模型。

This study aimed to develop the ideal procedure for cellular cardiomyoplasty for severe cardiac failure. Fetal cardiomyocyte (FCM) transplantation prevents but does not reverse cardiac remodeling in rats with chronic MI. However, prevascularization with bFGF-incorporated microspheres could enhance the benefits of FCM transplantation. FCM transplantation exerted preventive effects against late LV dilation and dysfunction after LV repair surgery.Autologous cells, such as skeletal myoblasts (SM) and bone marrow stem cells (BMSC), were more tolerant for ischemia as compared to CM. In the study of SM. transplantation to the MI heart, a large number of freshly-isolated neonatal SM can survive in the host heart and fully replace the infarcted myocardium with reverse LV remodelling in rats with MI. Intravenous (IV) and intramyocardial (IM) deliveries of BMSC transplantation. in mice were assessed echocardiographically and histologically in MI model and in dilated cardiomyopathy (DCM) model. Transplantation was performed either in acute or chronic phase. In acute phase, both IV and IM deliveries in BMSC transplantation were effective in both MI and DCM models, whereas in chronic phase, IM delivery was superior in. MI model, and IV administration was superior in DCM model.