Cardiomyocyte transplantation therapy for the congestive heart failure

本研究旨在评估心肌细胞移植作为心脏移植替代方案的潜在益处。在每一项研究中，我们进行了胚胎心肌细胞移植到大鼠慢性心肌梗死。<fetal cardiomyocyte transplantation >TX组收缩末期内径小于对照组，短轴缩短率优于对照组。TX组移植前后各项指标无明显变化。胎儿心肌细胞（FCM）移植只能预防但不能逆转慢性心肌梗死大鼠的心脏重构。< fCM transplantation with bFGF>本研究探讨了细胞移植前缺血区血管预形成的有效性。FGF组植入bFGF明胶微球，FGF-TX组先植入bFGF，再植入FCM。bFGF治疗后1周，瘢痕组织中出现新生血管。LV最大时变弹性在FGF-TX组中高于TX和FGF组。在组织学上，FGF-TX组中的移植细胞比TX组中存活更多。用bFGF掺入的微球预血管化增强了FCM移植的益处。< fCM transplantation with left ventricular plasty>47只左室动脉瘤大鼠接受了培养基注射（CMI）（n=10 ;组I）、FCM移植（CM-TX）（n=10 ;组II）、用CMI进行的左室修复（n=14 ;组III）或用胎儿CM-TX进行的左室修复（n=13 ;组IV），并在随后的4周内进行随访。在后期，IV组的LV尺寸小于III组，尽管两组最初显示出相似的降低。在最后一周，组IV的LVEmax是各组中最高的。CM-TX对左室修复术后晚期左室扩张和功能障碍有预防作用。

This study was designed to estimate the potential benefit of cardiomyocyte transplantation as an alternative to cardiac transplantation. In each study we performed fetal cardiomyocyte transplantation to the rats with chronic myocardial infarction. <fetal cardiomyocyte transplantation > TX group had smaller end-systolic dimension and better fractional shortening than Control group. However, there were no differences in all parameters before and after transplantation in TX group. Fetal cardiomyocyte (fCM) transplantation only prevented but did not reverse cardiac remodeling in rats with chronic myocardial infarction. < fCM transplantation with bFGF> This study investigate the efficacy of prevascularization in ischemic regions prior to cell transplantation. FGF group received the gelatin hydrogel microspheres incorporating bFGF, and FGF-TX group received bFGF pretreatment sequentially followed by fCM transplantation. Neovascularization was found in the scar tissue one week after bFGF treatment. LV maximum time-varying elastance was higher in the FGF-TX group than in the TX and FGF groups. Histologically, more transplanted cells survived in the FGF-TX group than in the TX group. Prevascularization with bFGF-incorporated microspheres enhances the benefits of fCM transplantation. < fCM transplantation with left ventricular plasty> Fourty-seven rats with LV aneurysm underwent culture medium injection (CMI) (n=10 ; group I), fCM transplantation (CM-TX) (n=10 ; group II), LV repair with CMI (n=14 ; group III), or LV repair with fetal CM-TX (n=13 ; group IV), and were followed-up during the subsequent 4 weeks. In the late period, LV dimension in group IV was smaller than those in group III, although they initially showed similar decreases in both groups. At the final week, LVEmax in group IV was the highest among the groups. CM-TX exerted preventive effects against late LV dilation and dysfunction after LV repair surgery.