Development of intraoperative monitoring system to map the higher cortical function of human brain using intrinsic optical imaging.

开发术中监测系统，利用内在光学成像绘制人脑的高级皮质功能。

• 批准号: 13470286
• 负责人: OHNO Kikuo
• 金额: $ 10.5万
• 依托单位: Tokyo Medical and Dental University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-13470286/
• 关键词: optical imaging; brain mappiong; positron emission tomography; temporal lobe epilepsy; intraoperative monitoring; somatosensory response; MEG; auditory response; 聴覚野

1) New intraoperative recording. apparatus (light source, analysis softoware and computer system) was ecquipped using this grant. By this, practically applicable intraopearteve real time monitoring system was established and usefully applied as guide in brain tumor surgery.2) From the point of neuroscience research of human brain, findings to support the hierarchically distributed human sensory system in cerebral cortex were obtained and the results were published. Also, detailed somatotopic representation map of hand area of human primary sensory cortex was obtained. In this, sensory response to the stimulus of each digit was recorded on the adjacent but different spots along the central sulcus. Such anatomic map is only obtainable with optical imaging.3) Comparison between optical recording, and MEG and PET vas performed using three dimensional image analyses. It revealed that the location of response specially corresponded along the axis of central sulcus but the actual three-dimensional location among gyrus was different.4) As the first step to record the higher cortical function, cortical response to pure tone stimuli was recorded in patients with left temporal glioma. In all patients, response was recorded on the supramarginal gyrus but not on the superior temporal gyrus, on which blood flow response was noted in the preoperative language stimulation. This finding suggested the different circuit between pure tone and language stimulation.5) A new project to apply the optical imaging to investigate the neuronal response of the human cerebral specimen was conducted. Cortical specimen was obtained in patients with intractable epilepsy and was incubated in camber to record the neuronal response to electrical stimulation using optical method. Recording of glucose metabolism in the same specimen was also performed. These projects are still on going but the preliminary results revealed the different characteristics between metabolic response and electrical response.

1)新的术中记录。仪器（光源、分析软件和计算机系统）是利用这笔赠款装备的。2）从人脑神经科学研究的角度，获得了支持大脑皮层感觉系统分层分布的发现，并发表了相关研究结果。获得了人初级感觉皮层手区的详细躯体位置表征图。在这种情况下，对每个手指刺激的感觉反应记录在中央沟沿着相邻但不同的点上。3）光学记录与MEG和PET三维图像分析的比较。结果显示，反应的位置沿中央沟轴方向沿着有特异性对应，但各回间的实际三维位置不同。4）记录左侧颞叶胶质瘤患者纯音刺激的皮层反应，作为记录高级皮层功能的第一步。在所有患者中，反应记录在缘上回，但没有在上级颞回，其上的血流反应，在术前的语言刺激。这一发现表明纯音和语言刺激之间存在着不同的回路。5）开展了一项新的应用光学成像技术研究人脑标本神经元反应的项目。取难治性癫痫患者大脑皮层标本，置于cambridge中孵育，用光学方法记录神经元对电刺激的反应。还记录了同一标本中的葡萄糖代谢。这些项目仍在进行中，但初步结果揭示了代谢反应和电反应之间的不同特征。

项目成果

Sato K, Nariai T, Ohno K et al.: "Intraoperative Intrinsinc Optical Imaging of Neuronal Activity from Subdivisions of the Human Primary Somatosensory Cortex"Cereb Cortex. 12. 269-280 (2002)

Sato K、Nariai T、Ohno K 等人：“人类初级体感皮层细分神经元活动的术中内在光学成像”大脑皮层。

Sasaki S, Yazawa I, Miyakawa N, Mochida H, Shinomiya K, Kamino K, Momose-Sato Y, Sato K.: "Optical imaging of intrinsic signals induced by peripheral nerve stimulation in the in vivo rat spinal cord."Neuroimage. 17. 1240-1255 (2002)

Sasaki S、Yazawa I、Miyakawa N、Mochida H、Shinomiya K、Kamino K、Momose-Sato Y、Sato K.：“体内大鼠脊髓周围神经刺激诱导的内在信号的光学成像。”神经图像。

Nariai T, Ohno K, et al.: "Intrinsic optical recording of somatrosensory responses in the human cerebral cortex during brain tumor surgery in Brain Activation and CBF Control"Tomita M, Kanno I, E H, eds. Brain activation and CBF control. Amsterdam : Elsev

Nariai T、Ohno K 等人：“大脑激活和 CBF 控制中脑肿瘤手术期间人类大脑皮层体感反应的内在光学记录”Tomita M、Kanno I、E H 编辑。

Duan L, Aoyagi M, Tamaki M, Yoshino Y, Morimoto T, Wakimoto H, Nagasaka Y, Hirakawa K, Ohno K, Yamamoto K: "Impairment of both apoptotic and cytoprotective signalings in glioma cells resistant to the combined use of cisplatin and tumor necrosis factor alp

Duan L、Aoyagi M、Tamaki M、Yoshino Y、Morimoto T、Wakimoto H、Nagasaka Y、Hirakawa K、Ohno K、Yamamoto K：“对顺铂和肿瘤联合使用产生耐药性的神经胶质瘤细胞中凋亡和细胞保护信号传导的损害

Ohta Y, Nariai T, Ishii K, Ishiwata K, Senda M, Okeda R, Ohno K, Hirakawa K.: "Meningio-angiomatosis in a patient with focal epilepsy : Value of pet in diagnoses and preoperative planning of surgery."Acta Neurochir (Wien). 145. 587-591 (2003)

Ohta Y、Nariai T、Ishii K、Ishiwata K、Senda M、Okeda R、Ohno K、Hirakawa K.：“局灶性癫痫患者的脑膜血管瘤病：宠物在诊断和术前手术计划中的价值。”Acta Neurochir