Molecular biological properties of oral treponemes and their outer membrane extracts

口腔密螺旋体及其外膜提取物的分子生物学特性

• 批准号: 13470390
• 负责人: OGAWA Tomohiko
• 金额: $ 7.1万
• 依托单位: Asahi University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-13470390/
• 关键词: Oral treponema; Treponema medium; Outer membrane; Human gingival eptithelial cell; Toll-like receptor; Periodontal disease; Immunobiological activity; Cytokine; ヒト歯肉上皮細胞

Oral treponemes are considered to be important in the development and progression of periodontal diseases. We investigated the recognition and activation mechanisms of human gingival epithelial cells (HGEC) with the oral treponemes Treponema denticola, Treponema vincentii, and Treponema medium, and their outer membrane extracts (OMEs). T. vincentii and T. medium but not T. denticola produced IL-8 in an HGEC culture. Further, all 3 treponemes induced IL-8 Mrna expression and NF-Kb activation in HGEC. Among them, T. denticola especially exhibited trypsin- and chymotrypsin- like protease activities, and the addition of chymostatin, a chymotrypsin protease inhibitor, resulted in detectable IL-8 production by HGEC cultured with T. denticola. Additionally, IL-8 Mrna expression in HGEC cultured with the 3 treponemes and their OMEs was definitely inhibited by the mouse anti-human Toll-like receptor (TLR) 2 monoclonal antibody, TL2.1. These findings suggest that oral treponemes and their OMEs activate human gingival epithelial cells through TLR2

口腔密螺旋体被认为在牙周病的发展和进展中是重要的。我们研究了口腔密螺旋体齿垢密螺旋体、文森特密螺旋体和密螺旋体培养基及其外膜提取物（OMEs）对人牙龈上皮细胞（HGEC）的识别和激活机制。T. vincentii和T. medium而不是T.在HGEC培养物中，齿垢产生IL-8。此外，所有3种密螺旋体均诱导HGEC中IL-8 mRNA表达和NF-κ B活化。其中T.特别是齿垢菌表现出胰蛋白酶和胰凝乳蛋白酶样蛋白酶活性，并且加入胰凝乳蛋白酶抑制剂chymostatin导致与齿垢菌一起培养的HGEC产生可检测的IL-8。齿垢此外，IL-8 mRNA的表达与3密螺旋体和它们的OME培养的HGEC明确抑制小鼠抗人Toll样受体（TLR）2单克隆抗体，TL2.1。这些发现表明，口腔密螺旋体及其OME通过TLR 2激活人类牙龈上皮细胞

项目成果

小川 知彦: "Porphyromonas gingivalis合成リピドAに対するTLR欠損マウス由来歯肉線維芽細胞の認識機構"エンドトキシン研究. 4. 73-80 (2001)

小川智彦：“来自 TLR 缺陷小鼠的牙龈成纤维细胞对牙龈卟啉单胞菌合成脂质 A 的识别机制”内毒素研究 4. 73-80 (2001)。

Yasuyuki Asai: "Detection and quantification of oral treponemes in subgingival plaque by real-time PCR"Journal of Clinical Microbiology. 40. 3334-3340 (2002)

Yasuyuki Asai：“通过实时 PCR 检测和定量龈下菌斑中的口腔密螺旋体”临床微生物学杂志。

Asai, Y. et al.: "Distribution and quantification of oral treponemas in subgingival plaque using real-time PCR"J. Clin. Microbiol. 40(9). 3334-3340 (2002)

Asai, Y. 等人：“使用实时 PCR 检测龈下菌斑中口腔密螺旋体的分布和定量”J.

Asai, Y. et al.: "Oral treponemes and their outer membrane extracts activate human gingival epithelial cells through Toll-like receptor 2"Infect. Immun. 71(2). 717-725 (2003)

Asai, Y. 等人：“口腔密螺旋体及其外膜提取物通过 Toll 样受体 2 激活人类牙龈上皮细胞”感染。

Yasuyuki Asai: "Oral treponemes and their outer membrane extracts activate human gingival epithelial cells through Toll-like receptor2"Infection and Immunity. 71・2. 717-725 (2003)

Yasuyuki Asai：“口腔密螺旋体及其外膜提取物通过 Toll 样受体 2 激活人类牙龈上皮细胞”感染与免疫 717-725 (2003)。