Chemical analysis and immunobiological activities of lipid A molecules derived from oral spirochete

本文研究了热酚-水法分离的密螺旋体培养液ATCC 700293的脂多糖（LPS）和Vogel等方法纯化的LPS（Rp-LPS）的内毒素性质和免疫生物学活性。T.medium LPS和Rp-LPS没有表现出或表现出非常低的内毒素活性，即半乳糖胺负载小鼠的致死毒性和鲎的活性。T.medium LPS和Rp-LPS对C_3H/HeN小鼠脾单个核细胞无促有丝分裂活性。T培养基LPS几乎不诱导人外周血单个核细胞产生白细胞介素-6（IL-6）。人牙龈上皮细胞也有少量IL-8产生。这些结果似乎与T.medium LPS水解不产生类脂A组分的观点相一致，T.medium的细胞壁与其它革兰氏阴性菌的结构特征有很大不同，LPS或其类脂A的替代物的化学分析值得进一步研究。氯仿-甲醇混合溶剂提取物经TLC分析得到单一斑点，用~ 1H-NMR谱鉴定为糖脂，用~ 1H-NMR谱鉴定了培养基乙醇提取物中的其他糖脂。为了阐明这些糖脂的化学性质和免疫生物学活性，需要进行更多的研究。

The endotoixic property and immunobiological activities of lipopolysaccharide(LPS)of Treponema medium ATCC 700293 by hot phenol-water method as well as the repurified LPS(Rp-LPS)by the method of Vogel et.al.were investigated. T.medium LPS and Rp-LPS exhibited no or very low endotoxic activities, that is, lethal toxicity in galactosamine-loaded mice and the activity of the Limulus tes. Tno mitogenic activity of T.medium LPS and Rp-LPS in C3H/HeN murine splenic mononuclear cells was also observed. Futher, T.medium LPS induced almost no production of interleukin-6(IL-6)in human peripheral blood mononuclear cells. A little production of IL-8 was also observed in human gingival epithelial cells. These results appear to coincide with the view that no lipid A fraction was yield by hydrolysis of T.medium LPS.The cell wall of T.medium seems to be quite different from structural feature of other gram-negative bacteria, and the chemical analysis of the substitute for LPS or its lipid A is worthy of further study. The extract of chloroform-methanol mixed solvent was purified to give a single spot by TLC analysis, and identified as a glycolipid using ^1H-NMR.Other grlycolipids including in the ethanol extract of T.medium were also identified by ^1H-NMR spectral data. To clarify the chemical properties and immunobiological activities of these glycolipids, much more research is needed.