Chemical analysis and immunobiological activities of lipid A molecules derived from oral spirochete

口腔螺旋体脂质 A 分子的化学分析和免疫生物学活性

• 批准号: 11671829
• 负责人: OGAWA Tomohiko
• 金额: $ 2.56万
• 依托单位: Asahi University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11671829/
• 关键词: Treponema medium; LPS; Glycolipid; Endotoxic property; Immunobiological activity; Cytokine; Chemical structure

The endotoixic property and immunobiological activities of lipopolysaccharide(LPS)of Treponema medium ATCC 700293 by hot phenol-water method as well as the repurified LPS(Rp-LPS)by the method of Vogel et.al.were investigated. T.medium LPS and Rp-LPS exhibited no or very low endotoxic activities, that is, lethal toxicity in galactosamine-loaded mice and the activity of the Limulus tes. Tno mitogenic activity of T.medium LPS and Rp-LPS in C3H/HeN murine splenic mononuclear cells was also observed. Futher, T.medium LPS induced almost no production of interleukin-6(IL-6)in human peripheral blood mononuclear cells. A little production of IL-8 was also observed in human gingival epithelial cells. These results appear to coincide with the view that no lipid A fraction was yield by hydrolysis of T.medium LPS.The cell wall of T.medium seems to be quite different from structural feature of other gram-negative bacteria, and the chemical analysis of the substitute for LPS or its lipid A is worthy of further study. The extract of chloroform-methanol mixed solvent was purified to give a single spot by TLC analysis, and identified as a glycolipid using ^1H-NMR.Other grlycolipids including in the ethanol extract of T.medium were also identified by ^1H-NMR spectral data. To clarify the chemical properties and immunobiological activities of these glycolipids, much more research is needed.

采用热酚-水法研究了密螺旋体培养基ATCC 700293的脂多糖(LPS)的内毒素性质和免疫生物学活性，以及​​采用Vogel等人的方法纯化的LPS(Rp-LPS)。 T.medium LPS 和 Rp-LPS 没有表现出或表现出非常低的内毒素活性，即半乳糖胺负载​​小鼠的致死毒性和鲎 tes 的活性。还观察到 T.medium LPS 和 Rp-LPS 在 C3H/HeN 鼠脾单核细胞中没有促有丝分裂活性。此外，T.medium LPS几乎不诱导人外周血单核细胞产生白细胞介素6(IL-6)。在人牙龈上皮细胞中也观察到少量产生IL-8。这些结果似乎与T.medium LPS水解没有产生脂质A组分的观点相一致。T.medium的细胞壁似乎与其他革兰氏阴性菌的结构特征有很大不同，LPS或其脂质A的替代品的化学分析值得进一步研究。将氯仿-甲醇混合溶剂提取物纯化，通过TLC分析得到单点，并使用 1 H-NMR鉴定为糖脂。T.培养基的乙醇提取物中包括的其他糖脂也通过 1 H-NMR光谱数据鉴定。为了阐明这些糖脂的化学性质和免疫生物学活性，需要进行更多的研究。

项目成果

Mastuguchi, T.: "Gene expressions of Toll-like receptor 2, but not Toll-like receptor 4, is induced by LPS and inflammatory cytokines in mouse macrophages"J, Immunol.. 165・10. 5767-5772 (2000)

Mastuguchi, T.：“Toll 样受体 2 的基因表达，而非 Toll 样受体 4，是由小鼠巨噬细胞中的 LPS 和炎症细胞因子诱导的”J，Immunol.. 165・10 (2000)。

Takeuchi,O.: "Cellular responses to bactersal all well components are mediated through Myp88-dependent signaling cascades"Int.Immunol.. 12・1. 113-117 (2000)

Takeuchi, O.：“细胞对细菌所有成分的反应都是通过 Myp88 依赖性信号级联介导的”Int. 12・1 (2000)。

Umemoto,T., et al.: "Fimbria-mediated coaggregation between human oral anaerobes Treponema medium and Porphyromonas gingivalis"Microbiol.Immunol. 43. 837-845 (1999)

Umemoto,T. 等人：“人口腔厌氧菌密螺旋体培养基和牙龈卟啉单胞菌之间菌毛介导的共聚集”Microbiol.Immunol。

Takechi, O.: "Cellular responses to bacterial cell wall components are mediated through MyD88-dependent signaling cascade"Int.Immunol.. 12・1. 113-117 (2000)

Takechi, O.：“细胞对细菌细胞壁成分的反应是通过 MyD88 依赖性信号级联介导的”Int.Immunol.. 12・1 (2000)。

Mokuno, Y.: "Expression of Toll-libe receptor 2 on JST ulls bearing in variant Vj6/V8l induced by Escherichia coli infection"J Immunol.. 165・2. 931-940 (2000)

Mokuno，Y.：“大肠杆菌感染诱导的变体Vj6/V8l中的Toll-libe受体2在JST ulls上的表达”JImmunol.. 165・2（2000）。