With the aim of expansion of the application of dental implants for the aged person and the patient who have systemic disease : the development of dental implants combined with the cell tranfected gene of bone related protein

本研究旨在扩大牙种植体在老年人和有全身性疾病患者稀疏骨修复中的应用。首先，我们研究了我们建立的表达成骨细胞的外源性骨桥蛋白(OP)和骨连接蛋白(ON)的黏附能力。Western blotting检测到外源性OP和ON的表达。将亲本Saos2、Saos2-Puro、Saos2-pcDNA3、Saos2-OP和Saos2-on以1.5×10~(-5)细胞密度接种于无血清DMEM中。分别于孵育6、12、24小时后计数细胞粘附率。无血清培养24小时后，测定细胞黏附剪切力(F)和细胞黏附面积(A)。Saos2-OP细胞的CAR高于亲本细胞(p&lt；0.05)，Saos2-On细胞的CAR低于亲本细胞(p&lt；0.05)。Saos2-OP细胞的F/A值高于亲本细胞，但差异无统计学意义。TH…更多的ESE结果表明，人OP是一种有效的成骨细胞样细胞黏附增强剂。其次，我们研究了糖尿病对骨整合后骨内种植体周围骨组织的影响。将钛种植体植入大鼠股骨。植入后8周，腹腔注射链脲佐菌素诱导糖尿病。进行组织病理学和组织形态计量学评价。糖尿病对骨-种植体接触率无明显影响，但对照组新生骨数量多于糖尿病组。接下来，我们开发了与骨髓细胞相结合的牙科植入物。未分化的骨髓细胞在含地塞米松的种植体上培养2周。将含有培养的骨髓细胞的种植体植入大鼠的皮下组织。进行组织病理学检查，并用RT-PCR进行分析。术后4周取牙种植体表面观察骨组织，RT-PCR检测骨钙素基因的表达。提示牙种植体联合骨蛋白基因转导细胞对老年人和有全身性疾病患者的稀疏骨有较好的疗效。较少

The purpose of this study was expansion of the application of dental implants for sparse bone of the aged person and the patient who have systemic disease. Firstly, we investigate the adhesive potential of exogeneous osteopontin(OP) and osteonectin(ON) expressing osteoblastic cells that we have established. The expression of exogeneous OP and ON was confirmed by Western blotting. Parental Saos2, Saos2-puro, Saos2-pcDNA3, and Saos2-OP and Saos2-ON were seeded on the polystyrene dish at a density of 1.5 x 10^5 cells in serum-free DMEM. The cell adhesion rates(CAR) were counted after 6, 12 and 24 hours of incubation. Also, the cell adhesive shear force (F) and cell adhesive area (A) were measured in serum-free DMEM after 24 hours. The CAR of Saos2-OP was higher than those of parental cells (p<0.05), and that of Saos2-ON was lower than those of parental cells (p<0.05). F/A of Saos2-OP had higher than the parental cells, though no significance was observed in the difference between them. Th … More ese results suggest that human OP is a potent enhancer of osteoblast-like cell adhesion. Secondary, we investigated the effect of diabetes on bone tissue around endosseous implants after osseointegration. Titanium implants were inserted in the femora of rats. At 8 weeks after implantation, diabetes was induced by intra peritoneal injection of streptozotocin. Histopathological and histomorphometric evaluations were carried out. There was no effect of diabetes on the ratio of bone-implant contact, while the amount of newly formed bone in the control group was greater than that of the diabetic group. Next, we developed dental implants combined with the bone marrow cells. Undifferentiated bone marrow cells were cultured on dental implants with dexamethasone for 2 weeks. Dental implants with cultured bone marrow cells were implanted into subcutaneous tissue in rats. Histopathogical investigation and analysis by RT-PCR were done. At 4 weeks after surgery, bone tissue was observed on the surface of dental implants and expression of mRNA of osteocalcin was detected by RT-PCR. These results suggested that the dental implants combined with the cells transfected gene of bone protein were effective on the sparse bone of the aged person and the patient who have systemic disease. Less