Experimental study on the application of calcium phosphate cement for endodontic treatment in pediatric dentistry.

磷酸钙骨水泥在儿童牙髓治疗中应用的实验研究

• 批准号: 13470447
• 负责人: TAKAGI Yuzo
• 金额: $ 6.34万
• 依托单位: Tokyo Medical and Dental University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-13470447/
• 关键词: Pulpotomy; Direct pulp capping; Calcium phosphate cemment; Self setting; Biomaterial; Dentin bridge; Endodontic Treatment; Pediatric Dentistry; 即時硬化性; 生活断髄糊剤; DCPD系硬化体; OCP硬化体; 水酸化アパタイト; 庇蓋硬組織形成

If there is a substance which has physical propaties favorable for use in dental material and biocompatibility, it can be more suitable for the dressing of pulpotomy than calcium hydroxide (CH). In the present study, biocompatibility. chemical stability, and ability to promote hard tissue formation of a new calcium phosphate cement (CPC) setting composed of octacalcium phosphate (OCP) and alpha-tricalcium phosphate (alpha-TCP) are examined using in vitro and in vivo assessment systems.In the first year, polarized hydroxyapatite crystals were implanted in to bone marrow of rat tibiae to examine its ability to promote hard tissue formation. The results showed that the abilities to promote hard tissue formation were different between positively charged surface and negatively charged surface. We, therefore, reserved to use in the new material. On the other hand, a CPC which was composed of OCP and alpha-TOP in 31 % and 69 % respectively showed physical propaties most favorable for use in dental material in vitro experiments. From second year of this project, therefore, this CPC selling was used as a pulp capping agent in rats, and the tissue reaction was examined histopathologically. The results showed that the reparative dentin formation was more prominent in OH used group in the early stage of the experiment. 5 weeks after pulp capping, however, formation of reparative dentin was prominent in the CPC setting group. Furthermore, reparative dentin consisting of regular dentinal tubules was observed more frequently in the OOP-based cement group. OCP-based cement may be useful in endodontic treatment because it allowed favorable healing processes to occur in the dental pulp.

如果有一种物质具有有利于牙科材料使用和生物相容性的物理性质，它可以比氢氧化钙(CH)更适合用于牙髓切断术的敷料。在本研究中，生物相容性。利用体外和体内评价系统检测了由磷酸八钙(OCP)和α-磷酸三钙(α-TCP)组成的新型磷酸钙骨水泥(CPC)的化学稳定性和促进硬组织形成的能力。第一年，将极化的羟基磷灰石晶体植入大鼠胫骨骨髓中，以检测其促进硬组织形成的能力。结果表明，正电荷表面和负电荷表面促进硬组织形成的能力不同。因此，我们保留在新材料中使用。另一方面，由OCP和α-TOP组成的CPC分别占31%和69%，显示出最适合用于牙科材料体外实验的物理微粒。因此，从本项目的第二年开始，该CPC被用作大鼠的盖髓剂，并对组织反应进行了组织病理学检查。结果表明，在实验的早期，羟基组修复性牙本质的形成更为明显。盖髓后5周，CPC组修复性牙本质形成明显。此外，在OOP粘固剂组中，修复性牙本质由规则的牙本质小管组成的频率更高。基于OCP的粘固剂可能在牙髓治疗中有用，因为它允许牙髓发生良好的愈合过程。

项目成果

N.C.Teng, S.Nakamura, Y Takagi, Y Yamashita, M.Ohgaki, K.Yamashita: "A new approach to enhancement of bone formation by electrically polarized hydroxyapatite."Journal of Dental Research. 80. 1925-1929 (2001)

N.C.Teng、S.Nakamura、Y Takagi、Y Yamashita、M.Ohgaki、K.Yamashita：“一种通过电极化羟基磷灰石增强骨形成的新方法。”牙科研究杂志。

M.Sena, Y.Yamashita, Y.Nakano, M.Ohgaki, S.Nakamura K.Yamashita, Y Takagi: "Octacalcium phosphate-based cement as a pulp capping agent inrats."Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics. (in press).

M.Sena、Y.Yamashita、Y.Nakano、M.Ohgaki、S.Nakamura K.Yamashita、Y Takagi：“八钙磷酸盐水泥作为大鼠盖髓剂。”口腔外科、口腔医学、口腔病理学、口腔

N.C.Teng: "A new approach to enhancement of bone formation by electrically polalized hydroxyapatite"Journal of Dental Research. 80. 1925-1929 (2001)

N.C.Teng：“一种通过电极化羟基磷灰石增强骨形成的新方法”牙科研究杂志。

M.Sena et al.: "Octacalcium phophate-based cement as a pulp capping agent inrats."Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics. (in press).

M.Sena 等人：“基于磷酸八钙的水泥作为大鼠盖髓剂。”口腔外科、口腔医学、口腔病理学、口腔放射学和牙髓病学。

M.Sena et al.: "Octacalcium phosphate-based cement as a pulp capping agent in rats."Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics. (in press).

M.Sena 等人：“八钙磷酸盐水泥作为大鼠盖髓剂。”口腔外科、口腔医学、口腔病理学、口腔放射学和牙髓学。