Induction of dentin-bridge formation after pulpotomy by biomaterials

生物材料诱导牙髓切断术后牙本质桥的形成

• 批准号: 62570912
• 负责人: TAKAGI Yuzo
• 金额: $ 1.34万
• 依托单位: Tokyo Medical and Dental University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1987
• 资助国家: 日本
• 起止时间: 1987 至 1988
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-62570912/
• 关键词: Pulpotomy; Induction; Dentin-bridge; Phosphophoryn; _2HS glycoprotein; 移植; 生活断髄; 象牙質; 石灰化

Non-collagenous matrix of dentin has been thought to play important role in the process of dentin mineralization. In this study, we determined whether non-collagenous dentin matrix can induce hard tissue formation in the subcutaneous tissue of experimental animals.When tissue distributions of dentin phosphophoryn and _2HS glycoprotein were examined by histochemical or immunohistochemical method, it was found that both of them were present in dentin being strongly associated with mineral crystals. The data suggested that these proteins might play role in the process of mineralization. Then, induction of hard tissue formation was attempted by subcutaneous implantation of these proteins into rats. When polyacrylamide gel disc containing dentin phosphophoryn were subcutaneously implanted into rat, mineral crystals were fromed in the gel disc. These results suggest that phosphophoryn may be able to induce the formation of dentin-bridge when it is placed on the surface of dental pulp after pulpotomy.

牙本质非胶原基质在牙本质矿化过程中起着重要作用。本研究用组织化学和免疫组织化学方法观察了牙本质磷酸化蛋白和_2HS糖蛋白的组织分布，发现两者均存在于牙本质中，并与矿物晶体密切相关。这些结果提示这些蛋白质可能在矿化过程中发挥作用。然后，通过将这些蛋白质皮下植入大鼠尝试诱导硬组织形成。将含有牙本质磷蛋白的聚丙烯酰胺凝胶盘植入大鼠皮下，在凝胶盘中形成矿物晶体。结果提示，磷酸化蛋白在活髓切断术后置于牙髓表面，可诱导牙本质桥的形成。

项目成果

Takagi Yuzo: Calcified Tissue International. 42. 97-103 (1988)

高木雄三：钙化组织国际。

Takagi,Yuzo: Journal of Oral Pathology. 17. 208-212 (1988)

Takagi，Yuzo：口腔病理学杂志。

高木裕三: 口腔病学会雑誌. 56. (1989)

高木雄三：口腔医学会杂志 56。（1989）

Y. Takagi; S. Sasaki: "A probable common disturbance in the early stage of odontoblast differentiation in Dentinogenesis Imperfecta" Journal of Oral Pathology. 17. 208-212 (1988)

Y.高木；

Takagi Yuzo.: Calcified Tissue International. 42. 97-103 (1988)

高木雄三：钙化组织国际。