Influence of Periodontal Pathogens to Periodontal Disease at childhood

牙周病原菌对儿童期牙周病的影响

• 批准号: 13470456
• 负责人: SASA Ryuji
• 金额: $ 4.54万
• 依托单位: SHOWA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-13470456/
• 关键词: PCR; P.gingivalis; A.actionmycetemcomitans; Periodontal Disease; Children; Periodontal Pathogen; 歯周疾患; ヒト; Porphyromonas gingivalis; Actinobacillus Actinomycetemcomitans; Porphromonas gingivalis; Actinobacillus actinomycetemcomitans; 中国人

The present study examined the presence or absence of four bacterial species Porphyromonas gingivalis (P.g.), Actinobacillus actinomycetemcomitans (A. a.), Pievotella intermedia (P.i.), and Bacteroides forsythus (B.f.) using PCR in the oral cavities of children, then investigated relationships between bacterial species and various clinical parameters.The Subjects were 37 physically healthy children who visited the Showa University Pediatric Dental Clinic. A dental plaque sample was collected from the mesiobuccal and labial surfaces to the marginal gingiva of the right maxillary central incisor (FDI 11) and the right maxillary first molar (FDI 16). Extracted DNA from plaque samples was used for PCR in this study. The amplification products were photographed under UV illumination. The following clinical parameters were also assessed: intraoral examination, probing depth (PD) and Bleeding On Probing (BOP).At FDI 11, detection rate for P.g. was most low, followed by B.f., A.a. and P.i. in descending order. At FDI 16, detection rate for P.g. was most low, followed by P.i., A.a. and B.f. S significant difference in the detection rate for B.f. was identified between FDI 11 and 16. In addition, detection rates for A.a., P.i. and B.f. were high in children with mixed dentition, but low in those with permanent dentition. Furthermore, regarding the relationship between bacterial species and BOP, it was not regarded between P.g. and BOP. Detection rates of A.a., P.i. and B.f. were higher for BOP(+) than for BOP(-). Detection rates of Bacteria related to Periodontal Disease in oral cavity of Japanese Children was higher in this study. The result suggest that it was need searching of high risk children for periodontal disease in childhood.

本研究检查了四种细菌种类牙龈牙龈（P.G.），放线杆菌静脉肌动物（A. a。），Pievotella Intermedia（P.I.）（P.I.）和细菌性疾病（B.F.访问了星大学儿科牙科诊所的孩子。从中颌骨和唇表面收集了牙齿样品，到右上颌中央门牙的边缘牙龈（FDI 11）和右上颌第一磨牙（FDI 16）。在这项研究中，将从斑块样品中提取的DNA用于PCR。放大产品是在紫外线照明下拍摄的。还评估了以下临床参数：口腔检查，探测深度（PD）和探测中的出血（BOP）。在FDI 11，P.G.检测率。最低，其次是B.F.，A.A。和P.I.按顺序下降。在外国直接投资16时，P.G.的检测率最低，其次是P.I.，A.A。和B.F. B B.F.的检测率有显着差异。在外国直接投资11和16之间确定。此外，A.A.，P.I。的检测率和B.F.具有混合牙齿的儿童高，但具有永久性牙齿的儿童。此外，关于细菌物种与BOP之间的关系，在P.G.之间没有被认为。和bop。 A.A.，P.I。的检测率和B.F. BOP（+）的比（ - ）高。在这项研究中，日本儿童口腔中与牙周疾病有关的细菌的检测率更高。结果表明，需要在儿童时期寻找高风险儿童以寻找牙周疾病。

项目成果

飯田綾子, 小林誠, 佐々龍二, 長谷川紘司, 他: "学校歯周病検診による歯周炎ハイリスク者スクリーニングの効果"昭和歯学会雑誌. 24(印刷中). (2004)

Ayako Iida、Makoto Kobayashi、Ryuji Sasa、Koji Hasekawa 等：“通过学校牙周病检查筛查高危牙周炎患者的效果”昭和牙科学会杂志 24（出版中）。

Ayako Iida, Makoto Kobayasi, Reiko Nanba, Taisuke Sato, Megumi Iwamaru, Yoshinori Hayashi, Takako Hiratsuka, Reiko Suda, Ryuji Sasa, Kouji Hasegawa: "Effect of Screening High Risk Patients of Periodontitis in Scool Periodontal Health Examination."The Jour

Ayako Iida、Makoto Kobayasi、Reiko Nanb​​a、Taisuke Sato、Megumi Iwamaru、Yoshinori Hayashi、Takako Hiratsuka、Reiko Suda、Ryuji Sasa、Kouji Hasekawa：“在学校牙周健康检查中筛查牙周炎高危患者的效果。”Jour

倪雪岩, 伊田博, 佐々龍二, 他: "PCRによる小児口腔内からの歯周病原性細菌の検出"昭和歯学会雑誌. 24(印刷中). (2004)

Niseiwa、Hiroshi Ida、Ryuji Sasa 等人：“通过 PCR 检测儿童口腔中的牙周病原菌”，昭和牙科学会杂志 24（出版中）。

Ni Xue Yan, Hiroshi Ida, Takeshi Igarashi, Nobuichi Goto, Motoyuki Suzuki, Koji Hasegawa, Mituko Inoue, Ryuji Sasa: "Detection of Bacteria related to Periodontal Disease in Oral Cavity of Japanese Children by Species-specific PCR."The Journal of Showa Uni

Ni Xue Yan、Hiroshi Ida、Takeshi Igarashi、Nobuichi Goto、Motoyuki Suzuki、Koji Hasekawa、Mituko Inoue、Ryuji Sasa：“通过物种特异性 PCR 检测日本儿童口腔中与牙周病相关的细菌。”昭和杂志