Effects of food consistency on expression of extracellular matrices in the temporo-mandibular joints

食物稠度对颞下颌关节细胞外基质表达的影响

• 批准号: 13470454
• 负责人: MIZOGUCHI Itaru
• 金额: $ 9.28万
• 依托单位: Health Sicence University of Hokkaido
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-13470454/
• 关键词: Temporomandibular joint; Extracellular matrix; Proteoglycan; Colllagen; Food consistency; RT-PCR; Western blotting; Immunohistochemistry; プロテオグリカン; Western Blotting; 顎関節円板; バーシカン; 成長変化; ノーザン・ハイブリダイゼーション; PCR; cDNA

To analyse the effects of food consistency on expression of extracellular matrix components in rat temporomandibular joint (TMJ), expression and localization of the core proteins of small reisin-rich proteoglycan and a large chondroitin sulphate proteoglycan, versican, in the TMJ discs during postnatal development (2 weeks to 32 weeks) were examined, using Western blotting, immunohistochemistry and rapid-cycle real-time RT-PCR. Western blotting analysis showed that protein expression was rapidly decreased after birth in biglycan, increased up to 16 weeks for decorin, and increased after birth, peaked at 8 weeks, and then gradually decreased for versican. An immunohistochemical study showed that staining for each proteoglycans was and homogeniously distributed in the TMJ discs at 2 weeks. The regional differences of the staining for became prominent after 4 weeks; the staining for decorin and biglycan was weak in the central part of the discs and intense in the anterior and posterior peripheral attachments, which would mainly experience tensile forces. The staining for biglycan was preferentially localized in the posterior band, on which compressive force would be exerted. mRNA expression was peak after birth and gradually decreased with growing. These results demonstrate that the growth-related changes and regional differences exist in the expression of versican in the TMJ discs of growing rats, which probably reflect the alteration of biomechanical environments caused by development of orofacial functions. Further study concerning the effects of food consistency would be necessary to understand mechanisms of temporomandibular joint diseases (TMD)s.

为了分析食物稠度对大鼠颞下颌关节（TMJ）细胞外基质成分表达的影响，采用免疫印迹、免疫组化和快速循环实时荧光定量RT-PCR方法，检测了大鼠出生后（2 ~ 32周）TMJ盘中富含小reisin的蛋白多糖和大硫酸软骨素蛋白多糖versican核心蛋白的表达和定位。蛋白质印迹分析表明，蛋白质表达迅速下降出生后，双糖蛋白聚糖，增加到16周的核心蛋白聚糖，出生后增加，在8周达到高峰，然后逐渐下降的多功能蛋白聚糖。免疫组织化学研究显示，在2周时，每种蛋白多糖的染色在TMJ盘中均匀分布。4周后，染色区域差异明显，核心蛋白聚糖和双糖链蛋白聚糖在椎间盘中央部分染色较弱，在前、后周边附件染色较强，主要受拉力作用。双糖链蛋白聚糖的染色优先定位在后带中，压缩力将施加在后带上。mRNA表达在出生后达到高峰，随生长发育逐渐下降。这些结果表明，生长期大鼠颞下颌关节盘中versican的表达存在生长相关性变化和区域性差异，可能反映了口面功能发育所引起的生物力学环境的改变。进一步研究食物稠度对颞下颌关节疾病的影响对于了解颞下颌关节疾病的发病机制是必要的。

项目成果

Takahashi I, Onodera K, Sasano Y, Mizoguchi I, Bae JW, Mitani H, Kagayama NI, Mitani H: "Effect of stretching on gene expression of β1 integrin and focal adhesion kinase and chondrogenesis through cell-extracellular matrix interactions."Eur J Cell Biol. 8

Takahashi I、Onodera K、Sasano Y、Mizoguchi I、Bae JW、Mitani H、Kagayama NI、Mitani H：“拉伸对 β1 整联蛋白和粘着斑激酶基因表达以及通过细胞-细胞外基质相互作用的软骨形成的影响。”Eur J细胞生物学8。

Hayama M: "Cell death of osteocytes occurs in rat alveolar bone during experimental tooth movement"Caloif Tissue Int. 70(2). 117-126 (2002)

Hayama M：“实验性牙齿移动期间，大鼠牙槽骨中发生骨细胞死亡”Caloif Tissue Int。

Takahashi I, Onodera K, 他: "Effects of stretching on gene expression of β1 integrin and focal adhesion kinase and chondrogenesis through cell-extracellular matrix."Eur J Cell Biol. 82(4). 182-192 (2003)

Takahashi I、Onodera K 等人：“拉伸对 β1 整联蛋白和粘着斑激酶的基因表达以及通过细胞外基质的软骨形成的影响。”Eur J Cell Biol 82(4) (2003)。

Abiko Y, Nishimura NI, Mitamura J, Mizoguchi I Rahemtulla F, Kaku T.: "Immunohistochemical localization of large chondroitin sulphate proteoglycan in porcine gingival epithelia."Eur J Morphol. 39. 99-104 (2001)

Abiko Y、Nishimura NI、Mitamura J、Mizoguchi I Rahemtulla F、Kaku T.：“猪牙龈上皮中大硫酸软骨素蛋白多糖的免疫组织化学定位。”Eur J Morphol。

Hamaya M, Takahashi I, Sasano Y, Kuwabara M, Iijima M, hayashi K, Yamazaki A, Shibata T, Kagayama NI, Mitani H, Mizoguchi I: "An immunohistochemical study of the localization of types I, II and III collagen in the temporomandibular joint of growing monkey

Hamaya M、Takahashi I、Sasano Y、Kuwabara M、Iijima M、hayashi K、Yamazaki A、Shibata T、Kagayama NI、Mitani H、Mizoguchi I：“I、II 和 III 型胶原蛋白定位的免疫组织化学研究