Transgenermonal mutations in the children of the liquidators who worked for the Chernobyl nuclear power plant accident

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To examine the transgenerational mutations by ionizing radiation at low dose and low dose rate in human, microsatellite length polymorphic mutations have been investigated in the F1 offspring of the liquidators who worked for the Chernobyl accident. Lymphocytes from peripheral blood were collected from the of exposed families (consisted of male liquidators living in Minsk and his partner and children) and from the unexposed families (consisted of fathers living in Minsk, uncontaminated area, with no history of liquidators and his partner and children whose age and sex were matched those of the children in exposed group) with the collaboration of Dr. Yelisseeva group. After the original lymphocytes were expanded, DNA was extracted and mutations were assayed. Final number of Fl offsprings was 62 and 85 for the exposed and unexposed control group, respectively. Twenty-one autosomal, one X linked and 21 Y linked microsarellites were used. For the autosomal microsatellite, mutation frequencies (/locus/gamete) were 0.64% (12/1858) and 0.80% (19/2362) in exposed and unexposed group, respectively. For the Y-linked microsatellites, mutation frequencies for the exposed and unexposed group were 0.44% (3/675) and 0.23% (2/835), respectively. This indicates that no difference of the mutation frequency between two group in the autosomal microsatellites. For the Y-linked microsatellites, tendency of higher mutation frequency has been seen in the exposed groups, though it is not significant. For the exposed group, 40 F1 children were from spermatogonial and 22 were from post spermatogonial exposure. The mutation frequency for the post spermatogonial was lower than that for the spermatogonial exposure.