Transgenermonal mutations in the children of the liquidators who worked for the Chernobyl nuclear power plant accident

切尔诺贝利核电站事故清算人子女的跨基因突变

• 批准号: 13480167
• 负责人: RYO Haruko
• 金额: $ 9.54万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-13480167/
• 关键词: Chernobyl; radionuclides contamination; genetic effects; lymphocytes; microsatellite; low dose・low dose rate; germ cell mutation; human mutation; 放射線; マイクロサテライト変異; 性細胞の変異

To examine the transgenerational mutations by ionizing radiation at low dose and low dose rate in human, microsatellite length polymorphic mutations have been investigated in the F1 offspring of the liquidators who worked for the Chernobyl accident. Lymphocytes from peripheral blood were collected from the of exposed families (consisted of male liquidators living in Minsk and his partner and children) and from the unexposed families (consisted of fathers living in Minsk, uncontaminated area, with no history of liquidators and his partner and children whose age and sex were matched those of the children in exposed group) with the collaboration of Dr. Yelisseeva group. After the original lymphocytes were expanded, DNA was extracted and mutations were assayed. Final number of Fl offsprings was 62 and 85 for the exposed and unexposed control group, respectively. Twenty-one autosomal, one X linked and 21 Y linked microsarellites were used. For the autosomal microsatellite, mutation frequencies (/locus/gamete) were 0.64% (12/1858) and 0.80% (19/2362) in exposed and unexposed group, respectively. For the Y-linked microsatellites, mutation frequencies for the exposed and unexposed group were 0.44% (3/675) and 0.23% (2/835), respectively. This indicates that no difference of the mutation frequency between two group in the autosomal microsatellites. For the Y-linked microsatellites, tendency of higher mutation frequency has been seen in the exposed groups, though it is not significant. For the exposed group, 40 F1 children were from spermatogonial and 22 were from post spermatogonial exposure. The mutation frequency for the post spermatogonial was lower than that for the spermatogonial exposure.

为了检测低剂量和低剂量率电离辐射对人类的跨代突变，对切尔诺贝利事故清算者的F1后代进行了微卫星长度多态突变的研究。外周血淋巴细胞采集自暴露家庭(由居住在明斯克的男性清盘人员及其配偶和子女组成)和非暴露家庭(由居住在明斯克未受污染地区的父亲、无清盘人员及其伴侣病史以及年龄和性别与暴露组儿童年龄和性别匹配的儿童组成)。原代淋巴细胞扩增后，提取DNA，进行突变检测。暴露组和未暴露对照组的最终F1子代数量分别为62和85。其中常染色体21个，X连锁1个，Y连锁21个。常染色体微卫星突变频率在暴露组为0.64%(12/1858)，非暴露组为0.80%(19/2362)。Y连锁微卫星突变频率暴露组为0.44%(3/675)，非暴露组为0.23%(2/835)。这说明两组常染色体微卫星的突变频率没有差异。Y连锁微卫星的突变频率在暴露组中有升高的趋势，但不显著。暴露组40名F1儿童来自精原细胞暴露，22名来自精原细胞暴露后。精原细胞暴露后突变频率低于精原细胞暴露。

项目成果

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Furitu, K. et al.: "Transgenerational mutations in the children of the liquidators who worked for the Chernobyl nuclear power plant accident"J.Radiat.Res.. 44(4). 420 (2003)

Furitu, K. 等人：“参与切尔诺贝利核电站事故的清算人子女的跨代突变”J.Radiat.Res. 44(4)。