Analysis of the function of CD9 and tetraspanins

CD9和四跨膜蛋白的功能分析

• 批准号: 13480238
• 负责人: MEKADA Eisuke
• 金额: $ 9.73万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-13480238/
• 关键词: membrane fusion; C. elegans; fertilization; epidermis; RNAi; complex; 膜4回貫通型; テトラスパニン; CD9; TM4ファミリー; ノックアウトマウス

(1) CD9-/-eggs are defect in fertilization, especially the fusion step with sperm.The fusion activity of CD9-/-eggs with sperm was recovered by the injection of CD9 mRNA into CD9-/-eggs. We constructed various mutant forms of CD9 mutants and examined their activities by injection their mRNA to CD9-/-eggs. These experiments suggestrd that the C-terminal domain of CD9 is essential for the recovery of fusion activity.(2) There are 29 members of the tetraspanins in C. elegans. We carried out RNA interference experiments for these tetraspanins to explore the role of tetraspanins in a whole animal. One of the tetraspanins, TSP-15, showed marked effect in body integrity. Present study opens new approach to analyse tetraspanins function by genetic methods.

(1)CD 9-/-卵子在受精过程中存在缺陷，尤其是与精子的融合过程，通过向CD 9-/-卵子中注射CD 9 mRNA，可恢复CD 9-/-卵子与精子的融合活性。我们构建了各种突变形式的CD 9突变体，并通过将它们的mRNA注射到CD 9-/-卵中来检测它们的活性。这些实验证实了CD 9的C-末端结构域对于融合活性的恢复是必不可少的。(2)在C.优雅的我们对这些四跨膜蛋白进行了RNA干扰实验，以探索四跨膜蛋白在整个动物中的作用。其中一种四跨膜蛋白TSP-15对机体的完整性有明显的作用。本研究为从遗传学角度分析四跨膜蛋白的功能开辟了新的途径。

项目成果

Kawashima, M., etc: "CD9 expression in solid non-neuroepithelial tumors and infiltrative astrocytic tumors"J.Histochem.Cytochem.. 50. 1195-1203 (2002)

Kawashima，M.等：“实体非神经上皮肿瘤和浸润性星形细胞肿瘤中的CD9表达”J.Histochem.Cytochem..50.1195-1203(2002)

Kusukawa, J. Ryu, F. Kameyama, T. Mekada, E.: "Reduced expression of CD9 in oral squamous cell carcinoma: CD9 expression inversely related to high prevalence of lymph node metastasis."J. Oral Pathol. Med.. 30. 73-79 (2001)

Kusukawa, J. Ryu, F. Kameyama, T. Mekada, E.：“口腔鳞状细胞癌中 CD9 表达降低：CD9 表达与淋巴结转移的高发生率呈负相关。”

Takenobu, H. Yamazaki, A. Hirata, M. Umata, T. and Mekada, E.: "The stress-and the inflammatory cytokine-indued ectodomain shedding of heparin-binding EGF-like growth factor is mediated by p38MAPK, distinct from TPA-induced and LPA-induced signaling casca

Takenobu, H. Yamazaki, A. Hirata, M. Umata, T. 和 Mekada, E.：“应激和炎症细胞因子诱导的肝素结合 EGF 样生长因子的胞外域脱落是由 p38MAPK 介导的，与

Shimizu, T., etc: "Elevated cerebrospinal fluid levels of anti-CD9 antibodies in patients with subacute sclerosing panencephalitis"J.Infect.Dis.. 185. 1346-1350 (2002)

Shimizu, T.等：“亚急性硬化性全脑炎患者脑脊液中抗CD9抗体水平升高”J.Infect.Dis.. 185. 1346-1350 (2002)

Saito, M., etc: "Diphtheria toxin receptor-mediated conditional and targeted cell ablation In transgenic mice"Nat. Biotechnol.. 19. 746-750 (2001)

Saito，M.等：“转基因小鼠中白喉毒素受体介导的条件性和靶向细胞消融”Nat。