Introduction of exogenous proteins into nucleus by using anthrax toxin

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Anthrax toxin is comprised of functionally different two components. Lethal factor (LF) is a effector molecule, while protective antigen (PA) is a molecule which facilitates the ently of LF into cytosol. The N-terminal part of LF molecule is binding domain to PA.Fusion proteins containing the N-terminal part of LF has been shown to be translocated to the cytosol by means of the activity of PA.In this study we attempted to make a recombinant proteins which were translocated into nuclei from outside of cells by interaction with PA.We have constructed the fusion proteins comprising GFP and N-terminal hexsa lysine complex. However, this protein was quite unstable and it did not permit to analyze the translocation of the fusion proteins. Constructions of other form of fusion proteins are in progress.