Identification and analysis of localizedmaternal determinants in ascidian egg cytoplasm

海鞘卵细胞质中局部母性决定因素的鉴定和分析

• 批准号: 13480245
• 负责人: NISHIDA Hiroki
• 金额: $ 9.54万
• 依托单位: Osaka University (2003)Tokyo Institute of Technology (2001-2002)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-13480245/
• 关键词: Ascidian; Early embryogenesis; Developmental fate; Maternal mRNA; Ooplasmic factor; EST database; Localized RNA; Cleavage pattern; ESTデータベース; cDNAプロジェクト

Maternal information within eggcytoplasm plays crucial roles in animal embryogenesis. In ascidians, the posterior-vegetal egg cytoplasm is involved in important events such as the antero-posterior axis specification, muscle and mesenchyme cell fate determination, germ cell formation, unequal cleavage, localization of specific maternal mRNAs, and cell cycle control. So far, we isolated nine localized maternal mRNAs and sequenced them. Then we carried out functional analysis of seven RNAs (HrPEM, HrPOPK-1, HrWnt-S, HrGLUT, HrPEM-3, HrPEN-1, HrPEM-2) by inhibition of translation with morpholino antisense oligonucleotides. The results demonstrated that these localized maternal mRNAs play various roles in embryogenesis. Especially, HrPEM (with no similarity) is involved in successive unequal cleavage in the posterior pole of embryos. Another localized mRNA, HrPOPK-1 (kinase), is required for concentration and relocalization of certain mRNAs to the centrosome-attracting body. These findings are interesting and worth for further extensive analysis.

细胞质内的母体信息在动物胚胎发生中起着至关重要的作用。在海鞘中，后植卵细胞质参与了许多重要的事件，如前后轴的形成、肌肉和间质细胞命运的决定、生殖细胞的形成、不平等分裂、特异性母体mrna的定位和细胞周期的控制。到目前为止，我们分离了9个定位的母体mrna并对其进行了测序。然后，我们对七个rna （HrPEM, HrPOPK-1, HrWnt-S, HrGLUT, HrPEM-3, HrPEN-1, HrPEM-2）进行了功能分析，通过抑制morpholino反义寡核苷酸翻译。结果表明，这些定位的母体mrna在胚胎发生过程中发挥着不同的作用。特别是，HrPEM（没有相似性）参与胚胎后极的连续不平等卵裂。另一种定位的mRNA， HrPOPK-1（激酶），是某些mRNA集中和重新定位到中心体吸引体所必需的。这些发现很有趣，值得进一步深入分析。

项目成果

Hiroki Nishida: "Specification of developmental fates in ascidian embryos : Molecular approach to maternal determinants and signaling molecules"International Review of Cytology. 217. 227-276 (2002)

Hiroki Nishida：“海鞘胚胎发育命运的规范：母体决定因素和信号分子的分子方法”国际细胞学评论。

Nishida, H.: "Spatio-temporal pattern of the activation of MAP kinase in embryos of the ascidian, Halocynthia roretzi"Dev.Growth Differ.. 45. 27-37 (2003)

Nishida, H.：“海鞘、Halocynthia roretzi 胚胎中 MAP 激酶激活的时空模式”Dev.Growth Differ.. 45. 27-37 (2003)

Makabe, K.: "Maternal genetic information stored in fertilized eggs of the ascidian, Halocynthia roretzi."Biology of Ascidian (eds.H.Sawada, H.Yokosawa, C.C.Lambert) (Springer-Verlag) (Tokyo). 165-177 (2001)

Makabe, K.：“海鞘受精卵中储存的母体遗传信息，Halocynthia roretzi。”海鞘生物学（eds.H.Sawada、H.Yokosawa、C.C.Lambert）（Springer-Verlag）（东京）。

Darras, S.: "The BMP/CHORDIN antagonism controls sensory pigment cell specification and differentiation in the ascidian embryo. Dev.Biol."Dev.Biol.. 236. 271-288 (2001)

Darras, S.：“BMP/CHORDIN 拮抗作用控制海鞘胚胎中的感觉色素细胞规格和分化。Dev.Biol.”Dev.Biol.. 236. 271-288 (2001)

Miya, T., Nishida, H.: "Expression pattern and transcriptional control of SoxB1 in embryos of the ascidian Halocynthia roretzi"Zool.Sci.. 20. 59-67 (2003)

Miya, T., Nishida, H.：“海鞘 Halocynthia roretzi 胚胎中 SoxB1 的表达模式和转录控制”Zool.Sci.. 20. 59-67 (2003)